Proteome analysis of mouse macrophages treated with anthrax lethal toxin

被引:25
作者
Chandra, H
Gupta, PK
Sharma, K
Mattoo, AR
Garg, SK
Gade, WN
Sirdeshmukh, R
Maithal, K
Singh, Y
机构
[1] Inst Genom & Integrat Biol, Delhi 110007, India
[2] Dr RML Avadh Univ, Faizabad, Uttar Pradesh, India
[3] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India
[4] Univ Delhi, Dr BR Ambedkar Ctr Biomed Res, Delhi 110007, India
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1747卷 / 02期
关键词
lethal toxin; Bacillus anthracis; proteomics; stress; ATP; cytoskeletal;
D O I
10.1016/j.bbapap.2004.10.012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Anthrax toxin produced by Bacillus anthracis is a tripartite toxin comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF). PA is the receptor-bindin component, which facilitates the entry of LF or EF into the cytosol. EF is a calmodulin-dependent adenylate cyclase that causes edema whereas LF is a zinc metalloprotease and leads to necrosis of macrophages. It is also important to note that the exact mechanism of LF action is still unclear. With this view in mind, in the present study, we investigated a proteome wide effect of anthrax lethal toxin (LT) on mouse macrophage cells (J774A.1). Proteome analysis of LT-treated and control macrophages revealed 41 differentially expressed protein spots, among which phosphoglycerate kinase 1, enolase 1, ATP synthase (beta subunit), tubulin beta2, gamma-actin, Hsp70, 14-3-3 zeta protein and tyrosine/tryptophan-3-monooxygenase were found to be down-regulated, while T-complex protein-1, vimentin, ERp29 and GRP78 were found to be up-regulated in the LT-treated macrophages. Analysis of up- and down-regulated proteins revealed that primarily the stress response and energy generation proteins play an important role in the LT-mediated macrophage cell death. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:151 / 159
页数:9
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