Taurine Buffers Glutamate Homeostasis in Retinal Cells in vitro under Hypoxic Conditions

被引：6

作者：

Chen, Fang ^{[1
]}

Mi, Mantian ^{[1
]}

Zhang, Qianyong ^{[1
]}

Wei, Na ^{[1
]}

Chen, Ka ^{[1
]}

Xu, Hongxia ^{[1
]}

Yuan, Jialin ^{[1
]}

Zhou, Yong ^{[1
]}

Lang, Haibin ^{[1
]}

Yu, Xiaoping ^{[1
]}

Wang, Bin ^{[1
]}

Wang, Jian ^{[1
]}

Tang, Yong ^{[1
]}

Chang, Hui ^{[1
]}

机构：

[1] Third Mil Med Univ, Chongqing Key Lab Nutr & Food Safety, Dept Nutr & Food Hyg, Chongqing 400038, Peoples R China

来源：

OPHTHALMIC RESEARCH | 2010年 / 44卷 / 02期

关键词：

Taurine; Glutamate; Retina; Muller cell; Hypoxia; ISOLATED RAT RETINA; GANGLION-CELLS; MULLER CELLS; GLIAL-CELLS; NITRIC-OXIDE; TRANSPORTERS; EXPRESSION; NEUROPROTECTION; SURVIVAL; RELEASE;

D O I：

10.1159/000312818

中图分类号：

R77 [眼科学];

学科分类号：

100212 [眼科学];

摘要：

Aims: We investigated whether taurine indirectly protects neurons under hypoxia by affecting retinal Muller cells, which are known to play important roles in the regulation of retinal glutamate content. Methods: Retinal cells isolated from rats were exposed to hypoxia for 24 h. We evaluated the retinal neuron survival, glutamate content in cultures with and without taurine under hypoxic conditions. The glutamate clearance function correlated with the expression of glutamine synthetase (GS) mRNA and L-glutamate/L-aspartate transporter (GLAST) mRNA. Immunohistochemical staining of glial fibrillary acidic protein (GFAP), vimentin and S-100 protein was performed to examine cytoskeletal changes in retinal Muller cells. Results: Retinal neurons treated with taurine exhibited significantly higher survival rates than those without taurine under hypoxia. Taurine inhibited the upregulation of GFAP and vimentin, and inhibited the downregulation of GLAST, GS and the nuclear-cytoplasmic ratio of S-100 under hypoxia. In addition, taurine inhibited the upregulation of the glutamate content in neurons and retinal Muller cells upon hypoxic exposure. Conclusion: These data suggest that hypoxic damage to cultured retinal cells is decreased by taurine. The neuroprotection by taurine may relate to buffering glutamate homeostasis via modulation of the glutamate clearance by retinal Muller cells. Copyright (C) 2010 S. Karger AG, Basel

引用

页码：105 / 112

页数：8

共 26 条

[1]

Exogenous glutamate and taurine exert differential actions on light-induced release of two endogenous amino acids in isolated rat retina [J].