Identification of genes regulated by prolonged acid exposure in Helicobacter pylori

被引：27

作者：

Dong, QJ ^{[1
]}

Hyde, D

Herra, C

Kean, C

Murphy, P

O'Morain, CA

Buckley, M

机构：

[1] Univ Ireland Trinity Coll, St James Hosp, Dept Clin Microbiol, Dublin 8, Ireland

[2] Univ Dublin Trinity Coll, Meath & Adelaide Hosp, Dept Clin Microbiol, Dublin 24, Ireland

[3] Univ Dublin Trinity Coll, Meath & Adelaide Hosp, Dept Gastroenterol, Dublin 24, Ireland

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 196卷 / 02期

关键词：

differential display polymerase chain reaction; acid stress; Helicobacter pylori;

D O I：

10.1016/S0378-1097(01)00076-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To investigate the influence of prolonged acid exposure on the gene expression, transcripts of Helicobacter pylori, grown under pH 5.5 and pH 7.4 for five successive passages, were analysed by differential display PCR. Eight genes were regulated by prolonged acid exposure. These genes included topA, tufB, ureB, flaA, atoE in the H. pylori genome and a cDNA fragment with 54% identity of the predicted amino acid sequence to a Bacillus cereus YkoW protein. The remaining two cDNA fragments had no significant homology to known sequences. Our data suggest that most of these genes might be required for the resistance of H. pylori to prolonged acid exposure. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：245 / 249

页数：5

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