Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

被引：71

作者：

Degano, Irene R. ^{[1
]}

Vilalta, Marta ^{[1
]}

Bago, Juli R. ^{[1
]}

Matthies, Annette M. ^{[2
,3
]}

Hubbell, Jeffrey A. ^{[2
,3
]}

Dimitriou, Helen ^{[4
]}

Bianco, Paolo ^{[5
]}

Rubio, Nuria ^{[1
]}

Blanco, Jeronimo ^{[1
]}

机构：

[1] Hosp Santa Creu & Sant Pau, CSIC, ICCC, Cardiovasc Res Ctr, Barcelona 08025, Spain

[2] Ecole Polytech Fed Lausanne, Inst Bioengn, CH-1015 Lausanne, Switzerland

[3] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland

[4] Univ Crete, Sch Med, Dept Pediat Hematol Oncol, GR-71003 Iraklion, Greece

[5] Univ Roma La Sapienza, Dept Expt Med & Pathol, I-00161 Rome, Italy

来源：

BIOMATERIALS | 2008年 / 29卷 / 04期

关键词：

in vivo imaging; cell differentiation; scaffold; luciferase; enhanced green fluorescent protein (eGFP);

D O I：

10.1016/j.biomaterials.2007.10.006

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

A combined strategy using bioluminescence imaging, bone densitometry and histology was used to analyze the bone regeneration capacity of human bone marrow (hBMSC) and adipose tissue (hAMSC) mesenchymal stem cells, seeded in an osteoconductive arginine-glycine-aspartate (RGD) crosslinked hydrogel scaffold, implanted in a mouse calvarial bone defect. We show that firefly luciferase labeled stem cells can be monitored in vivo through a prolonged 90 days period, during which hBMSCs survive better than hAMSCs and that the density of scaffold bearing defects increased significantly more than that of defects without scaffolds. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：427 / 437

页数：11

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