Mapping of genes involved in glutathione, carbohydrate and COR14b cold induced protein accumulation during cold hardening in wheat

被引:14
作者
Galiba, G [1 ]
Kerepesi, I
Vágújfalvi, A
Kocsy, G
Cattivelli, L
Dubcovsky, J
Snape, JW
Sutka, J
机构
[1] Hungarian Acad Sci, Agr Res Inst, H-2462 Martonvasar, Hungary
[2] Janus Pannonius Univ, Dept Analit & Struct Chem, H-7601 Pecs, Hungary
[3] Expt Inst Cereal Res, Sect Fiorenzuola Arda, Fiorenzuola Darda, Italy
[4] Univ Calif Davis, Dept Agron & Range Sci, Davis, CA 95616 USA
[5] John Innes Ctr Plant Sci Res, Cereal Res Dept, Norwich NR4 7UJ, Norfolk, England
关键词
carbohydrate; COR-protein; frost resistance; gene mapping; glutathione;
D O I
10.1023/A:1017532725385
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Using some of the chromosome substitution lines developed from the crosses of the donor Cheyenne to Chinese Spring we showed that the accumulation of water soluble carbohydrates during different stages of hardening was time dependent. Moreover there was a significant correlation between the rate of carbohydrate accumulation and the frost tolerance. The expression and regulation of a wheat gene homologous to the barley cold regulated cor14b gene was compared in frost sensitive and frost tolerant wheat genotypes at different temperatures. Studies made with chromosome substitution lines showed that the threshold induction temperature polymorphism of the cor14b wheat homologous gene was controlled by loci located on chromosome 5A of wheat, while cor14b gene was mapped, in Triticum monococcum, onto the long arm of chromosome 2A(m). Our study on the effect of cold hardening on glutathione (GSH) metabolism showed that chromosome 5A of wheat has an influence on the GSH accumulation and on the ratio of reduced and oxidised glutathione as part of a complex regulatory function during cold hardening. In addition, the level of increase in GSH content during hardening may indicate the degree of the frost tolerance of wheat.
引用
收藏
页码:173 / 177
页数:5
相关论文
共 21 条
[1]   MOLECULAR-CLONING AND CHARACTERIZATION OF COLD-REGULATED GENES IN BARLEY [J].
CATTIVELLI, L ;
BARTELS, D .
PLANT PHYSIOLOGY, 1990, 93 (04) :1504-1510
[2]   The interaction between cold and light controls the expression of the cold-regulated barley gene cor14b and the accumulation of the corresponding protein [J].
Crosatti, C ;
de Laureto, PP ;
Bassi, R ;
Cattivelli, L .
PLANT PHYSIOLOGY, 1999, 119 (02) :671-680
[3]   Genetic analysis of the accumulation of COR14 proteins in wild (Hordeum spontaneum) and cultivated (Hordeum vulgare) barley [J].
Crosatti, C ;
Nevo, E ;
Stanca, AM ;
Cattivelli, L .
THEORETICAL AND APPLIED GENETICS, 1996, 93 (5-6) :975-981
[4]  
Dubcovsky J, 1996, GENETICS, V143, P983
[5]   LINKAGE RELATIONSHIPS AMONG STRESS-INDUCED GENES IN WHEAT [J].
DUBCOVSKY, J ;
LUO, MC ;
DVORAK, J .
THEORETICAL AND APPLIED GENETICS, 1995, 91 (05) :795-801
[6]   Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signalling [J].
Foyer, CH ;
LopezDelgado, H ;
Dat, JF ;
Scott, IM .
PHYSIOLOGIA PLANTARUM, 1997, 100 (02) :241-254
[7]   RFLP MAPPING OF THE VERNALIZATION (VRN1) AND FROST-RESISTANCE (FR1) GENES ON CHROMOSOME 5A OF WHEAT [J].
GALIBA, G ;
QUARRIE, SA ;
SUTKA, J ;
MORGOUNOV, A ;
SNAPE, JW .
THEORETICAL AND APPLIED GENETICS, 1995, 90 (7-8) :1174-1179
[8]  
Galiba G., 1994, Plant Breeding Reviews, V12, P115
[9]   Location of a gene regulating cold-induced carbohydrate production on chromosome 5A of wheat [J].
Galiba, G ;
Kerepesi, I ;
Snape, JW ;
Sutka, J .
THEORETICAL AND APPLIED GENETICS, 1997, 95 (1-2) :265-270
[10]   Cold-regulated gene expression during winter in frost tolerant and frost susceptible barley cultivars grown under field conditions [J].
Giorni, E ;
Crosatti, C ;
Baldi, P ;
Grossi, M ;
Marè, C ;
Stanca, AM ;
Cattivelli, L .
EUPHYTICA, 1999, 106 (02) :149-157