HLA class II DNA typing in Buerger's disease

被引:14
作者
Aerbajinai, W
Tsuchiya, T
Kimura, A
Yasukochi, Y
Numano, F
机构
[1] TOKYO MED & DENT UNIV,MED RES INST,DEPT TISSUE PHYSIOL,BUNKYO KU,TOKYO 113,JAPAN
[2] TOKYO MED & DENT UNIV,DEPT INTERNAL MED 3,BUNKYO KU,TOKYO 113,JAPAN
关键词
Buerger's disease; HLA-DNA typing; PCR-RFLP; PCR-SSOP;
D O I
10.1016/S0167-5273(96)88790-0
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To analyze the genetic factors involved in the pathogenesis of Buerger's disease, the polymorphisms of HLA class II genes (HLA-DP, -DQ and -DRB) were investigated in 36 patients using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. HLA class (B54 and B52) and HLA class II (DQ alleles) genes were also typed by polymerase chain reaction-sequence-specific oligonucleotide probe (PCR SSOP) method to confirm alleles. PCR-RFLP typing revealed that the frequencies of HLA class II genes, DRB1*0405, DQB1*0401, DQA1*03 and DPB1*0501, were significantly increased in the patients with Buerger's disease compared with the healthy controls. Further, DNA typing confirmed that HLA-B54 was associated with Buerger's disease. These results suggested that the haplotype of HLA-B54-DRB1*0405-DQB1*0401- DQA1*03-DPB1*0501 was associated with Buerger's disease in Japanese population.
引用
收藏
页码:S197 / S202
页数:6
相关论文
共 24 条
[1]  
BAUER MP, 1980, HISTOCOMPATIBILITY T, P955
[3]  
DEMOERLOOSE P, 1979, TISSUE ANTIGENS, V14, P169
[4]  
ERLICH HA, 1990, PCR PROTOCOLS, P261
[5]   GENERATION OF SINGLE-STRANDED-DNA BY THE POLYMERASE CHAIN-REACTION AND ITS APPLICATION TO DIRECT SEQUENCING OF THE HLA-DQA LOCUS [J].
GYLLENSTEN, UB ;
ERLICH, HA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7652-7656
[6]  
HALDANE JBS, 1956, ANN HUM GENET, V20, P309
[7]  
INOKO H, 1993, HDB HLA TYPING TECHN, P9
[8]  
KIMURA A, IN PRESS INT J CARDI
[9]  
KJELDSEN K, 1969, ACTA CHIR SCAND, V135, P495
[10]   A SIMPLE AND RAPID METHOD FOR HLA-DP GENOTYPING BY DIGESTION OF PCR-AMPLIFIED DNA WITH ALLELE-SPECIFIC RESTRICTION ENDONUCLEASES [J].
MAEDA, M ;
URYU, N ;
MURAYAMA, N ;
ISHII, H ;
OTA, M ;
TSUJI, K ;
INOKO, H .
HUMAN IMMUNOLOGY, 1990, 27 (02) :111-121