Confocal Laser Scanning Microscopy Method for Quantitative Characterization of Silica Monolith Morphology

被引：68

作者：

Bruns, Stefan ^{[1
]}

Muellner, Tibor ^{[1
]}

Kollmann, Martin ^{[2
]}

Schachtner, Joachim ^{[2
]}

Hoeltzel, Alexandra ^{[1
]}

Tallarek, Ulrich ^{[1
]}

机构：

[1] Univ Marburg, Dept Chem, D-35032 Marburg, Germany

[2] Univ Marburg, Dept Biol, D-35032 Marburg, Germany

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 15期

关键词：

POROUS POLYMER MONOLITHS; DOUBLE-PORE SILICA; STATIONARY PHASES; 3-DIMENSIONAL OBSERVATION; STRUCTURAL FORMATION; PACKED CAPILLARIES; COLUMNS; PERFORMANCE; SIZE; SEPARATION;

D O I：

10.1021/ac100909t

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a fast, nondestructive, and quantitative approach to characterize the morphology of capillary silica-based monolithic columns by reconstruction from confocal laser scanning microscopy images. The method comprises column pretreatment, image acquisition, image processing, and statistical analysis of the image data. The received morphological data are chord length distributions for the bulk macropore space and skeleton of the silica monolith. The morphological information is shown to be comparable to that derived from transmission electron microscopy, but far easier to access. The approach is generally applicable to silica-based capillary columns, monolithic or particulate. It allows the rapid acquisition of hundreds of longitudinal and cross-sectional images in a single session, resolving a multitude of morphological details in the column.

引用

页码：6569 / 6575

页数：7

共 66 条

[1] Mercury intrusion porosimetry and image analysis of cement-based materials [J].