Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches

被引：78

作者：

Brown, J

Brown, T

Fox, KR

机构：

[1] Univ Southampton, Sch Biol Sci, Div Biochem & Mol Biol, Southampton SO16 7PX, Hants, England

[2] Univ Southampton, Dept Chem, Southampton SO16 5NH, Hants, England

来源：

BIOCHEMICAL JOURNAL | 2001年 / 354卷

关键词：

DNA mismatches; DNA recognition; mismatch repair;

D O I：

10.1042/0264-6021:3540627

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT > GT > GG > AA approximate to TT approximate to TC > CA > GA > CC > GC, We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n) > (GC)(n) > A(n)T(n) > (AT)(n). Studies with base analogues show good binding to phiT (where phi represents 1',2'-dideoxy-ribose), but much weaker binding to G phi.

引用

页码：627 / 633

页数：7

共 38 条

[1] Nearest-neighbor thermodynamics of internal A•C mismatches in DNA:: Sequence dependence and pH effects [J].