Double-stranded RNA homopolymer Poly(rC)•Poly(rG) for a new pH-Sensitive drug carrier

被引：6

作者：

Asayama, Shoichiro ^{[1
]}

Ogawa, Ai ^{[1
]}

Kawakami, Hiroyoshi ^{[1
]}

Nagaoka, Shoji ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Dept Appl Chem, Tokyo 1920397, Japan

来源：

MOLECULAR PHARMACEUTICS | 2008年 / 5卷 / 01期

关键词：

poly(rC)center dot poly(rG); double-stranded RNA homopolymer; pH-sensitive drug carrier; endosomal pH; anticancer drug;

D O I：

10.1021/mp7001076

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Double-stranded RNA homopolymer poly(rC)center dot poly(rG) has been used as a new pH-sensitive drug carrier. The poly(rC)center dot poly(rG) had proton buffering capacity around pH 6, owing to protonation of cytosine, as determined by acid-base titration. By circular dichroism measurement, the protonation caused conformational change of the RNA. The poly(rC)center dot poly(rG) and doxorubicin (Dox), as an anticancer drug, formed the complexes which released the drugs at endosomal pH. The resulting complex exhibited higher anticancer activity than the Dox alone. These results result suggest that the poly(rC)center dot poly(rG) is a promising biopolymer for a new class of pH-sensitive drug carriers.

引用

页码：162 / 164

页数：3

共 10 条

[1] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[2]

CARTLIDGE S A, 1987, Journal of Controlled Release, V4, P253, DOI 10.1016/0168-3659(87)90017-4

[3] Factors affecting acoustically triggered release of drugs from polymeric micelles [J].

Husseini, GA ;

Myrup, GD ;

Pitt, WG ;

Christensen, DA ;

Rapoport, NAY .

JOURNAL OF CONTROLLED RELEASE, 2000, 69 (01) :43-52

[4] The binding of DNA intercalating and non-intercalating compounds to A-form and protonated form of poly(rC)•poly(rG):: Spectroscopic and viscometric study [J].

Sinha, R ;

Islam, MM ;

Bhadra, K ;

Kumar, GS ;

Banerjee, A ;

Maiti, M .

BIOORGANIC & MEDICINAL CHEMISTRY, 2006, 14 (03) :800-814

[5] Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes [J].

Sonawane, ND ;

Szoka, FC ;

Verkman, AS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (45) :44826-44831

[6] ENDOCYTOSIS AND THE RECYCLING OF PLASMA-MEMBRANE [J].

STEINMAN, RM ;

MELLMAN, IS ;

MULLER, WA ;

COHN, ZA .

JOURNAL OF CELL BIOLOGY, 1983, 96 (01) :1-27

[7] Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cells [J].

Thomas, M ;

Klibanov, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14640-14645

[8]

Unsworth J. M., 2003, J BIOMED MATER RES, V53, P617

[9]

YOKOYAMA M, 1991, CANCER RES, V51, P3229

[10] STUDIES ON CONFORMATION OF PROTONATED DNA [J].

ZIMMER, C ;

LUCK, G ;

VENNER, H .

BIOPOLYMERS, 1968, 6 (04) :563-+

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