Engineering tocopherol biosynthetic pathway in lettuce

被引：17

作者：

Li, Y. ^{[1
,2
]}

Wang, G. ^{[3
]}

Hou, R. ^{[1
]}

Zhou, Y. ^{[1
]}

Gong, R. ^{[3
]}

Sun, X. ^{[1
]}

Tang, K. ^{[1
,3
]}

机构：

[1] Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Shanghai 200433, Peoples R China

[2] Chinese Acad Sci, Shanghai Informat Ctr Life Sci, Shanghai 200031, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Agr & Biol, Plant Biotechnol Res Ctr, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Shanghai 200030, Peoples R China

来源：

BIOLOGIA PLANTARUM | 2011年 / 55卷 / 03期

关键词：

homogentisate phytyltransferase; Lactuca sativa; gamma-tocopherol methyltransferase; transformation; vitamin E; VITAMIN-E CONTENT; HOMOGENTISATE PHYTYLTRANSFERASE; SATIVA L; ARABIDOPSIS; EXPRESSION; PLANTS; METHYLTRANSFERASE; OVEREXPRESSION; MANIPULATION; DISSECTION;

D O I：

10.1007/s10535-011-0110-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In order to increase tocopherol content, genes encoding Arabidopsis homogentisate phytyltransferase (HPT) and gamma-tocopherol methyltransferase (gamma-TMT) were constitutively over-expressed in lettuce (Lactuca sativa L. var. logifolia), alone or in combination. Over-expression of hpt could increase total tocopherol content, while over-expression of gamma-tmt could shift tocopherol composition in favor of alpha-tocopherol. Transgenic lettuce lines expressing both hpt and gamma-tmt produced significantly higher amount of tocopherol and elevated alpha-/gamma-tocopherol ratio compared with non-transgenic control and transgenic lines harboring a single gene (hpt or gamma-tmt). The best line produced eight times more tocopherol than the non-transgenic control and more than twice than hpt single-gene transgenic line.

引用

页码：453 / 460

页数：8

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