Protein-protein interaction between the transcriptional repressor E4BP4 and the TBP-binding protein Dr1

被引：30

作者：

Cowell, IG ^{[1
]}

Hurst, HC ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,IMPERIAL CANC RES FUND,ONCOL UNIT,LONDON W12 ONN,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 18期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/24.18.3607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously mapped a repression domain from the active transcriptional repressor E4BP4 to a 65 amino acid segment near the C-terminus of the polypeptide. Here we show that the E4BP4 repression domain interacts specifically with the TBP binding repressor protein Drl. Mutants that affect the ability of E4BP4 to bring about transcriptional repression are also deficient in their binding of Drl. The results are discussed in the light of evidence for squelching of a 'global' repressor by a DNA binding defective E4BP4 mutant.

引用

页码：3607 / 3613

页数：7

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