Convective assembly of bacteria for surface-enhanced Raman scattering

被引:124
作者
Kahraman, Mehmet [1 ]
Yazici, M. Muege [1 ]
Sahin, Fikirettin [1 ]
Culha, Mustafa [1 ]
机构
[1] Yeditepe Univ, Fac Engn & Architecture, Genet & Bioengn Dept, Istanbul, Turkey
关键词
D O I
10.1021/la702240q
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A sample preparation method based on convective assembly for "whole-microorganism" identification using surface-enhanced Raman scattering (SERS) is developed. With this technique, a uniform sample can easily be prepared with silver nanoparticles. During the deposition process, bacteria and nanoparticles are assembled to form a unique well-ordered structure with great reproducibility. The SERS spectra acquired from the samples prepared with this technique have better quality and improved reproducibility for SERS spectra obtained from the same sample and limited variation due to the consistent sample preparation. E. coli, a Gram-negative bacilli, and Staphylococcus cohnii, a Gram-positive coccus, are studied as model bacteria.
引用
收藏
页码:894 / 901
页数:8
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