Genetic analysis of kernel hardness in bread wheat using PCR-based markers

被引:39
作者
Galande, AA
Tiwari, R
Ammiraju, JSS
Santra, DK
Lagu, MD
Rao, VS
Gupta, VS
Misra, BK
Nagarajan, S
Ranjekar, PK [1 ]
机构
[1] Natl Chem Lab, Div Biochem Sci, Plant Mol Biol Unit, Pune 411008, Maharashtra, India
[2] Directorate Wheat Res, Karnal 132001, India
[3] Agharkar Res Inst, Pune 411004, Maharashtra, India
关键词
wheat; kernel hardness (KH); quantitative trait loci (QTL); RAPD; ISSR; recombinant inbred lines (RILs);
D O I
10.1007/PL00002915
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait, 100 recombinant inbred lines (RILs) derived from a cross between a hard grain land-race, NP4, and a soft grain variety, HB 208, were screened with 100 ISSR and 360 RAPD primers. Eighteen markers were assigned to seven linkage groups covering 223.6 cM whereas I I markers remained unlinked. A multiple-marker model explained the percentage of phenotypic variation for kernel hardness as 20.6%, whereas that for protein content, hectolitre weight and 1,000-kernel weight was 18.8%, 13.5% and 12.1 %, respectively. Our results indicate that phenotypic expression of kernel hardness is controlled by many QTLs and is interdependent on various related traits.
引用
收藏
页码:601 / 606
页数:6
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