Expression of TRAIL and its receptors in human brain tumors

被引:78
作者
Frank, S
Köhler, U
Schackert, G
Schackert, HK
机构
[1] NINDS, Surg Neurol Branch, Biochem Sect, NIH, Bethesda, MD 20892 USA
[2] Tech Univ Dresden, Dept Surg Res, Dresden, Germany
关键词
apoptosis; TRAIL; TRAIL receptors; death receptors; signaling; brain neoplasms;
D O I
10.1006/bbrc.1999.0493
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recently, TRAIL has been demonstrated to selectively induce apoptosis in transformed cell lines, and subsequently four receptors (TRAIL-R1-TRAIL-R4) have been identified. The ability to transduce death signals is restricted to TRAIL-R1/TRAIL-R2. In contrast, TRAIL-R3/TRAIL-R4 are unable to activate apoptotic pathways and have therefore been suggested to act as "decoys" protecting normal tissues from cell death, However, the biological role of the TRAIL system remains incompletely understood. We analyzed the expression of TRAIL and its receptors in a panel of human brain tumors (n = 34) and in four glioma cell lines in comparison to normal brain tissue. Constant co-expression of TRAIL and of receptors TRAIL-R1, TRAIL-R2, and TRAIL-R3 in different tumor entities as well as in normal brain indicates that additional mechanisms might modulate the previously proposed "decoy" model. Furthermore, in contrast to previous reports, we demonstrate TRAIL and TRAIL-R2 to be present on a transcriptional level in normal brain tissue. Exceptional expression of TRAIL-R4 transcripts does not suggest a significant regulatory role of this receptor in the human brain and its tumors. (C) 1999 Academic Press.
引用
收藏
页码:454 / 459
页数:6
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