Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation

被引：10

作者：

Barre, FX

Mir, LM

Lécluse, Y

Harel-Bellan, A

机构：

[1] Inst Rech Canc, CNRS UPR 9079, F-94801 Villejuif, France

[2] Inst Gustave Roussy, CNRS UMR 1772, Villejuif, France

[3] Inst Gustave Roussy, CNRS UMR 1156, Villejuif, France

来源：

BIOTECHNIQUES | 1998年 / 25卷 / 02期

关键词：

D O I：

10.2144/98252gt04

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.

引用

页码：294 / 296

页数：3

共 9 条

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