Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics

被引:427
作者
Beretta, G
Granata, P
Ferrero, M
Orioli, M
Facino, RM
机构
[1] Univ Milan, Fac Farm, Ist Chim Farmaceut Tossicol, I-20131 Milan, Italy
[2] Univ Milan, Fac Farm, Scuola Specializzaz Sci & Technol Cosmet, I-20131 Milan, Italy
关键词
standardization; honey; antioxidants; optical methods; multivariate analysis;
D O I
10.1016/j.aca.2004.11.010
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The aim of this work was to establish a solid platform of analytical information for the definition/standardization of the antioxidant properties of honey. We investigated first the antioxidant/radical scavenging capacity of 14 commercial honeys of different floral and geographic origin, using a battery of spectrophotometric tests: Folin-Ciocalteu assay for phenol content (PC), ferric reducing antioxidant power (FRAP assay) for total antioxidant activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay for antiradical activity, absorbance:450 (ABS(450)) for color intensity and one fluorimetric method: ORAC, oxygen reactive antioxidant capacity for the antilipoperoxidant activity. Then the data from different procedures were compared and analysed by multivariate techniques (correlation matrix calculation, principal component analysis (PCA)). Significant correlations were obtained for all the antioxidant markers (r ranging from 0.933 to 0.716), with antioxidant properties strictly correlated to the phenolic content and honey color intensity. PCA found different clusters of honey based on the antioxidant power and very likely also on chemical composition. The results of this study demonstrated that only through a combination of antioxidant testings, comparative analyses, and chemometric evaluation we can achieve a strictly rigorous guideline for the characterization of the antioxidant activity of honey, an invaluable tool for the understanding/demonstration of its antioxidants linked therapeutic efficacy. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:185 / 191
页数:7
相关论文
共 27 条
[1]   A review of the analytical methods to determine the geographical and botanical origin of honey [J].
Anklam, E .
FOOD CHEMISTRY, 1998, 63 (04) :549-562
[2]  
[Anonymous], 1979, APIACTA, DOI DOI 10.1038/NCOMMS3838.5
[3]   Antioxidative effect of Maillard reaction products formed from honey at different reaction times [J].
Antony, SM ;
Han, IY ;
Rieck, JR ;
Dawson, PL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (09) :3985-3989
[4]   Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins [J].
Azeredo, LD ;
Azeredo, MAA ;
de Souza, SR ;
Dutra, VML .
FOOD CHEMISTRY, 2003, 80 (02) :249-254
[5]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[6]  
Bogdanov S, 1997, FOOD SCI TECHNOL-LEB, V30, P748, DOI 10.1006/fstl.1997.0259
[7]  
Brand-Williams W., 1995, WISS TECHNOL, V22, P25
[8]   Substituting honey for refined carbohydrates protects rats from hypertriglyceridemic and prooxidative effects of fructose [J].
Busserolles, J ;
Gueux, E ;
Rock, E ;
Mazur, A ;
Rayssiguier, Y .
JOURNAL OF NUTRITION, 2002, 132 (11) :3379-3382
[9]   Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships [J].
Cao, GH ;
Sofic, E ;
Prior, RL .
FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (05) :749-760
[10]  
CAVANAGH D, 1970, Journal of Obstetrics and Gynaecology of the British Commonwealth, V77, P1037