A mutation in the gene involved in sister chromatid separation causes a defect in nuclear mRNA export in fission yeast

被引：6

作者：

Azad, AK

Ideue, T

Ohshima, Y

Tani, T ^{[1
]}

机构：

[1] Kumamoto Univ, Fac Sci, Dept Biol Sci, Kumamoto 8608555, Japan

[2] Kyushu Univ, Grad Sch, Fac Sci, Dept Biol, Fukuoka 8128581, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 310卷 / 01期

关键词：

Ptr4p; Cut1p; mRNA export; chromosome segregation; fission yeast;

D O I：

10.1016/j.bbrc.2003.08.135

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fission yeast ptr4-1 is one of the mRNA transport mutants that accumulate poly(A)(+) RNA in the nuclei at the nonpermissive temperature. We cloned the ptr4(+) gene and found that it is identical with the cut1(+) gene essential for chromosome segregation during mitosis. ptr4/cut1 has no defects in nucleocytoplasmic transport of a protein, indicative of a specific blockage of mRNA export by this mutation. A mutant of Cut2p cooperating with Cut1p in sister chromatid separation also showed defective mRNA export at the nonpermissive temperature. Our results suggest a novel linkage between the cell division cycle and nuclear mRNA export in eukaryotic cells. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：176 / 181

页数：6

共 29 条

[1] ISOLATION AND CHARACTERIZATION OF RAT1 - AN ESSENTIAL GENE OF SACCHAROMYCES-CEREVISIAE REQUIRED FOR THE EFFICIENT NUCLEOCYTOPLASMIC TRAFFICKING OF MESSENGER-RNA
AMBERG, DC
GOLDSTEIN, AL
COLE, CN
[J]. GENES & DEVELOPMENT, 1992, 6 (07) : 1173 - 1189
[2] Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe
Azad, AK
Tani, T
Shiki, N
Tsuneyoshi, S
Urushiyama, S
Ohshima, Y
[J]. MOLECULAR BIOLOGY OF THE CELL, 1997, 8 (05) : 825 - 841
[3] Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation
Babu, JR
Jeganathan, KB
Baker, DJ
Wu, XS
Kang-Decker, N
van Deursen, JM
[J]. JOURNAL OF CELL BIOLOGY, 2003, 160 (03) : 341 - 353
[4] A MUTATION IN THE SCHIZOSACCHAROMYCES-POMBE RAE1 GENE CAUSES DEFECTS IN POLY(A)(+) RNA EXPORT AND IN THE CYTOSKELETON
BROWN, JA
BHARATHI, A
GHOSH, A
WHALEN, W
FITZGERALD, E
DHAR, R
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (13) : 7411 - 7419
[5] Nuclear RNA export
Cullen, BR
[J]. JOURNAL OF CELL SCIENCE, 2003, 116 (04) : 587 - 597
[6] Fission yeast cut1 and cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes
Funabiki, H
Kumada, K
Yanagida, M
[J]. EMBO JOURNAL, 1996, 15 (23) : 6617 - 6628
[7] ISOLATION AND CHARACTERIZATION OF SCHIZOSACCHAROMYCES-POMBE CUT MUTANTS THAT BLOCK NUCLEAR DIVISION BUT NOT CYTOKINESIS
HIRANO, T
FUNAHASHI, S
UEMURA, T
YANAGIDA, M
[J]. EMBO JOURNAL, 1986, 5 (11) : 2973 - 2979
[8] HOU Z, 2000, NATURE, V407, P401
[9] The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability
Jimeno, S
Rondón, AG
Luna, R
Aguilera, A
[J]. EMBO JOURNAL, 2002, 21 (13) : 3526 - 3535
[10] A CONDITIONAL YEAST MUTANT DEFICIENT IN MESSENGER-RNA TRANSPORT FROM NUCLEUS TO CYTOPLASM
KADOWAKI, T
ZHAO, YM
TARTAKOFF, AM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) : 2312 - 2316

← 1 2 3 →