A systematic analysis of the factors that determine the strength of pre-mRNA splicing enhancers

被引：139

作者：

Graveley, BR ^{[1
]}

Hertel, KJ ^{[1
]}

Maniatis, T ^{[1
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

来源：

EMBO JOURNAL | 1998年 / 17卷 / 22期

关键词：

pre-mRNA splicing; RNA looping; splicing enhancers; SR proteins;

D O I：

10.1093/emboj/17.22.6747

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We find that the strength of splicing enhancers is determined by the relative activities of the bound serine-arginine (SR)-rich splicing factors, the number of SR proteins within the enhancer complex and the distance between the enhancer and the intron. Remarkably, the splicing activity of the bound SR proteins is directly proportional to the number of RS tetrapeptide sequences within the RS domain. Quantitative analysis of the effects of varying the distance between the enhancer and the intron revealed that the splicing efficiency is directly proportional to the calculated probability of a direct interaction between the enhancer complex and the 3' splice site. These data are consistent with a model in which splicing enhancers function by increasing the local concentration of SR proteins in the vicinity of the nearby intron through RNA looping.

引用

页码：6747 / 6756

页数：10

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