Characterization of a poplar NIMA-related kinase PNek 1 and its potential role in meristematic activity

被引：34

作者：

Cloutier, M ^{[1
]}

Vigneault, F ^{[1
]}

Lachance, D ^{[1
]}

Séguin, A ^{[1
]}

机构：

[1] Nat Resources Canada, Canadian Forestry Serv, Laurentian Forestry Ctr, Ste Foy, PQ G1V 4C7, Canada

来源：

FEBS LETTERS | 2005年 / 579卷 / 21期

基金：

加拿大自然科学与工程研究理事会;

关键词：

NIMA-related kinase; cell cycle progression; meristems; higher plants;

D O I：

10.1016/j.febslet.2005.07.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Meristems are sites of undifferentiated cell division, which carry on developing into functional organs. Using the two-hybrid system with a poplar 14-3-3, we uncovered poplar NIMA-related kinase 1 (PNek1) as an interacting protein. PNek1 shows high homology to the mammalian NIMA-related kinases, which are thought to be involved in cell cycle progression. Using a synchronized poplar cell suspension, we observed an accumulation of PNek1 mRNA at the GIN transition and throughout the G(2)-to-M progression. Moreover, PNek1-GFP fusion protein localized in the cytoplasm and in both the nuclear and nucleolar regions. Overexpression of PNek1-GFP in Arabidopsis caused morphological abnormalities in flower and siliques. Overall, these results suggest that PNek1 is involved in plant development. Crown copyright (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. All rights reserved.

引用

页码：4659 / 4665

页数：7

共 48 条

[1]

Andersen JS, 2002, CURR BIOL, V12, P1, DOI 10.1016/S0960-9822(01)00650-9

[2] Cell cycle:: the key to plant growth control? [J].