Microspore embryogenesis in Apiaceae

被引：20

作者：

Ferrie, A. M. R. ^{[1
]}

Bethune, T. D. ^{[1
]}

Mykytyshyn, M. ^{[1
]}

机构：

[1] CNR, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2011年 / 104卷 / 03期

关键词：

Caraway (Carum carvi L.); Dill (Anethum graveolens L.); Doubled haploid; Embryogenesis; Fennel (Foeniculum vulgare Mill.); Microspore culture; ANTHER CULTURE; BRASSICA-CAMPESTRIS; NICOTIANA-TABACUM; HAPLOID PLANTS; INDUCTION; POLLEN; GROWTH; REGENERATION; REQUIREMENTS; PROTOPLASTS;

D O I：

10.1007/s11240-010-9770-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Haploid technology is used to develop uniform, true-breeding lines, as well as to accelerate crop improvement programs. Among 20 Apiaceae species screened for response to doubled haploidy, 11 generated microspore-derived embryos, and all but one of the latter yielded doubled haploid plants. Donor plant conditions, basal media, and culture conditions were evaluated for their efficacy on inducing microspore-derived embryos. Growing donor plants at temperature conditions of 10/5 or 15/10A degrees C promoted microspore embryogenesis in fennel (Foeniculum vulgare Mill.), whereas, growing donor plants at a temperature regime of 10/5A degrees C, along with the use of a cold extraction method, enhanced embryogenesis in dill (Anethum graveolens L.) and anise (Pimpinella anisum L.). The culture of fennel and dill microspores in an NLN basal medium and caraway (Carum carvi L.) in AT-3 basal medium promoted the highest frequencies of embryo induction.

引用

页码：399 / 406

页数：8

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