Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway

被引：52

作者：

Li, Hualing ^{[1
,2
,3
,4
,5
]}

Lv, Bei ^{[1
]}

Kong, Ling ^{[1
]}

Xia, Jing ^{[1
]}

Zhu, Ming ^{[1
]}

Hu, Lijuan ^{[1
]}

Zhen, Danyang ^{[1
]}

Wu, Yifan ^{[1
]}

Jia, Xiaoqin ^{[1
]}

Zhu, Sujuan ^{[6
]}

Cui, Hengmi ^{[2
,4
,5
,7
]}

机构：

[1] Yangzhou Univ, Med Coll, Dept Biochem, Yangzhou, Jiangsu, Peoples R China

[2] Jiangsu Key Lab Integrated Tradit Chinese & Weste, Yangzhou 225001, Jiangsu, Peoples R China

[3] Yangzhou Univ, Jiangsu Key Lab Zoonosis, Yangzhou, Jiangsu, Peoples R China

[4] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

[5] Yangzhou Univ, Inst Epigenet & Epigen, 88 South Univ Ave, Yangzhou 225009, Jiangsu, Peoples R China

[6] Yangzhou Univ, Biosci & Biotechnol Coll, Dept Biochem, 88 South Univ Ave, Yangzhou 225009, Jiangsu, Peoples R China

[7] Jiangsu Key Lab Expt & Translat Noncoding RNA Res, Yangzhou 225009, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2017年 / 40卷 / 04期

基金：

美国国家科学基金会;

关键词：

neuro-oncological ventral antigen 1; resistance; hypoxia; apoptosis; pathway; FACTOR-INDUCED INCREASE; CHRONIC MILD HYPOXIA; ALZHEIMERS-DISEASE; BRAIN; EXPRESSION; BINDING; GLYCOPROTEIN; INFLAMMATION; ISCHEMIA; EXPOSURE;

D O I：

10.3892/ijmm.2017.3089

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Neuro-oncological ventral antigen 1 (Nova1) is a well known brain-specific splicing factor. Several studies have identified Nova1 as a regulatory protein at the top of a hierarchical network. However, the function of Nova1 during hypoxia remains poorly understood. This study aimed to investigate the protective effect of Nova1 against cell hypoxia and to further explore the Bax/Bcl-2/caspase-3 pathway as a potential mechanism. During hypoxia, the survival rate of pheochromocytoma PC12 cells was gradually decreased and the apoptosis rate was gradually increased, peaking at 48 h of hypoxia. At 48 h after transfection of PC12 cells with pCMV-Myc-Nova1, the expression of Nova1 was significantly increased, with wide distribution in the cytoplasm and nucleus. Moreover, the survival rate was significantly increased and the apoptosis rate was significantly decreased. Additionally, the mRNA and protein expression levels of Bax and caspase-3 were significantly increased in the pCMV-Myc group and significantly decreased in the pCMV-Myc-Nova1 group, whereas that of Bcl-2 was significantly decreased in the pCMV-Myc group and significantly increased in the pCMV-Myc-Nova1 group. This study indicated that Nova1 could be linked to resistance to the hypoxia-induced apoptosis of PC12 cells via the Bax/Bcl-2/caspase-3 pathway, and this finding may be of significance for exploring novel mechanisms of hypoxia and the treatment of hypoxia-associated diseases.

引用

页码：1125 / 1133

页数：9

共 48 条

[1]

Hypoxia inducible factor 1α down regulates cell surface expression of α1,2-fucosylated glycans in human pancreatic adenocarcinoma cells [J].