Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II

被引：33

作者：

Bushnell, DA ^{[1
]}

Cramer, P ^{[1
]}

Kornberg, RD ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

STRUCTURE | 2001年 / 9卷 / 01期

关键词：

D O I：

10.1016/S0969-2126(00)00554-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protocol for the incorporation of SeMet into yeast proteins is described. Incorporation at a level of about 50% suffices for the location of Se sites in an anomalous difference Fourier map of the 0.5 MDa yeast RNA polymerase II. This shows the utility of the approach as an aid in the model-building of large protein complexes.

引用

页码：R11 / R14

页数：4

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