A simple method for cardiac surgery in rats

被引:5
作者
Connold, AL
Frischknecht, R
Vrbova, G
机构
[1] UNIV LONDON UNIV COLL,DEPT ANAT & DEV BIOL,LONDON WC1E 6BT,ENGLAND
[2] UNIV GENEVA,HOP CANTONAL,DEPT PHYS MED & REEDUC,CH-1211 GENEVA 4,SWITZERLAND
关键词
cardiac surgery; transplantation; embryonic cardiomyocytes; Fisher rats;
D O I
10.1016/0963-6897(95)02044-6
中图分类号
Q813 [细胞工程];
学科分类号
摘要
In our laboratory we have developed a relatively simple method for cardiac surgery in rats, The operation is carried out through a small incision in the chest wall using inexpensive equipment. This method allows for the delivery of tissue fragments and cells from a donor rat to an intact or damaged area of ventricular myocardium of a host rat, with easy subsequent localisation of the transplanted/grafted tissue. The rats recover well after the surgery and survive for long periods of time. The technique could also be used for the direct injection of chemicals or molecular probes into the heart. In our experiments we have found that embryonic rat cardiomyocytes that have been transplanted into adult host rat ventricular myocardium using this method survive and develop characteristics typical of heart muscle, thus indicating that using this technique the host heart offers a favourable environment for the transplanted embryonic heart cells.
引用
收藏
页码:405 / 409
页数:5
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