Surface-enhanced fluorescence on SiO2-coated silver island films

被引:103
作者
Tarcha, PJ
DeSaja-Gonzalez, J
Rodriguez-Llorente, S
Aroca, R
机构
[1] Abbott Labs, Hosp Prod Div, Abbott Pk, IL 60064 USA
[2] Univ Windsor, Mat & Surface Sci Grp, Windsor, ON N9C 3P4, Canada
关键词
surface-enhanced fluorescence; immunosensor; silicon dioxide coating; silicon oxide coating; silver island film; fluorescence quantum yield; surface-enhanced spectroscopy and photodecomposition; surface-enhanced resonance Raman scattering; Langmuir-Blodgett film;
D O I
10.1366/0003702991945443
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Fluorescence is one of the molecular spectroscopic properties that is enhanced by placing the molecule on the rough surface of a coinage metal. The surface-enhanced fluorescence (SEF) can be directly observed in steady-state fluorescence experiments, The observations are the results of a delicate balance between the enhanced emission and the quenching due to energy transfer to nonradiative surface plasmons, In the present report, SiO2-coated silver films were fabricated at varying dielectric thickness. The surface of the films was analyzed with the use of atomic force microscopy (AFM) and Xray photoelectron spectroscopy (XPS), AFM confirms the surface roughness and XPS analysis indicates that the SiO2 coverage was successful. SEF and SERS (surface-enhanced Raman scattering) were observed on active 6, 10, and 14 nm silver films coated with SiO2. Similar results were obtained with a 6 nm silver film coated with 6 nm SiO. The SEF work was carried out on fluorescent molecules with different quantum yield, and the typical enhancement factor obtained for the fluorescent signal was approximately 10, Both the SiO2 and SiO overlayers provide stable surfaces with well-defined hydrophilic properties, Such stable constructions have applicability towards the advancement of SERS and SEF as routine analytical techniques in bio- and chemical sensors.
引用
收藏
页码:43 / 48
页数:6
相关论文
共 16 条
  • [1] AROEA R, 1988, LANGMUIR, V4, P518
  • [2] Chance R. R., 1978, ADV CHEM PHYS, V37, P1, DOI DOI 10.1002/9780470142561.CH1
  • [3] Surface-enhanced fluorescence and SERRS spectra of N-octadecyl-3,4:9,10-perylenetetracarboxylic monoanhydride on silver island films
    DeSajaGonzalez, J
    Aroca, R
    Nagao, Y
    DeSaja, JA
    [J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 1997, 53 (02) : 173 - 181
  • [4] SERS OF LANGMUIR-BLODGETT MONOLAYERS - N-OCTYL-3,4-9,10-PERYLENETETRACARBOXYLIC MONOANHYDRIDE AND MONOIMIDE
    GUHATHAKURTAGHOSH, U
    AROCA, R
    LOUTFY, RO
    NAGAO, Y
    [J]. JOURNAL OF RAMAN SPECTROSCOPY, 1989, 20 (12) : 795 - 800
  • [5] SPECTROSCOPIC PROPERTIES AND PACKING OF LANGMUIR-BLODGETT MONOLAYERS OF PERYLENETETRACARBOXYLIC ANHYDRIDES
    JOHNSON, E
    AROCA, R
    [J]. LANGMUIR, 1995, 11 (05) : 1693 - 1700
  • [6] ENHANCED DYE FLUORESCENCE OVER SILVER ISLAND FILMS - ANALYSIS OF THE DISTANCE DEPENDENCE
    KUMMERLEN, J
    LEITNER, A
    BRUNNER, H
    AUSSENEGG, FR
    WOKAUN, A
    [J]. MOLECULAR PHYSICS, 1993, 80 (05) : 1031 - 1046
  • [7] Characterization of SiO2-overcoated silver-island films as substrates for surface-enhanced Raman scattering
    Lacy, WB
    Williams, JM
    Wenzler, LA
    Beebe, TP
    Harris, JM
    [J]. ANALYTICAL CHEMISTRY, 1996, 68 (06) : 1003 - 1011
  • [8] SURFACE ENHANCED SPECTROSCOPY
    METIU, H
    [J]. PROGRESS IN SURFACE SCIENCE, 1984, 17 (3-4) : 153 - 320
  • [9] SURFACE-ENHANCED RAMAN-SCATTERING OF ADSORBATES
    OTTO, A
    [J]. JOURNAL OF RAMAN SPECTROSCOPY, 1991, 22 (12) : 743 - 752
  • [10] IMMUNOASSAY EMPLOYING SURFACE-ENHANCED RAMAN-SPECTROSCOPY
    ROHR, TE
    COTTON, T
    FAN, N
    TARCHA, PJ
    [J]. ANALYTICAL BIOCHEMISTRY, 1989, 182 (02) : 388 - 398