Interaction with PCNA is essential for yeast DNA polymerase η function

被引：170

作者：

Haracska, L ^{[1
]}

Kondratick, CM ^{[1
]}

Unk, I ^{[1
]}

Prakash, S ^{[1
]}

Prakash, L ^{[1
]}

机构：

[1] Univ Texas, Med Branch, Sealy Ctr Mol Sci, Galveston, TX 77555 USA

来源：

MOLECULAR CELL | 2001年 / 8卷 / 02期

关键词：

D O I：

10.1016/S1097-2765(01)00319-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In both yeast and humans, DNA polymerase (Pol) eta functions in error-free replication of ultraviolet-damaged DNA, and Pol eta promotes replication through many other DNA lesions as well. Here, we present evidence for the physical and functional interaction of yeast Pol eta with proliferating cell nuclear antigen (PCNA) and show that the interaction with PCNA is essential for the in vivo function of Pol eta. Pol eta is highly inefficient at inserting a nucleotide opposite an abasic site, but interaction with PCNA greatly stimulates its ability for nucleotide incorporation opposite this lesion. Thus, in addition to having a pivotal role in the targeting of Pol eta to the replication machinery stalled at DNA lesions, interaction with PCNA would promote the bypass of certain DNA lesions.

引用

页码：407 / 415

页数：9

共 51 条

[1] SPECIFIC COMPLEX-FORMATION BETWEEN YEAST RAD6 AND RAD18 PROTEINS - A POTENTIAL MECHANISM FOR TARGETING RAD6 UBIQUITIN-CONJUGATING ACTIVITY TO DNA-DAMAGE SITES [J].

BAILLY, V ;

LAMB, J ;

SUNG, P ;

PRAKASH, S ;

PRAKASH, L .

GENES & DEVELOPMENT, 1994, 8 (07) :811-820

[2] Yeast DNA repair proteins Rad6 and Rad18 form a heterodimer that has ubiquitin conjugating, DNA binding, and ATP hydrolytic activities [J].

Bailly, V ;

Lauder, S ;

Prakash, S ;

Prakash, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (37) :23360-23365

[3] Enzymes and reactions at the eukaryotic DNA replication fork [J].

Bambara, RA ;

Murante, RS ;

Henricksen, LA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (08) :4647-4650

[4] p21(Cip1/Waf1) disrupts the recruitment of human Fen1 by proliferating-cell nuclear antigen into the DNA replication complex [J].

Chen, JJ ;

Chen, S ;

Saha, P ;

Dutta, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (21) :11597-11602

[5]

Creighton S, 1995, METHOD ENZYMOL, V262, P232

[6] Efficient and accurate replication in the presence of 7,8-dihydro-8-oxoguanine by DNA polymerase η [J].

Haracska, L ;

Yu, SL ;

Johnson, RE ;

Prakash, L ;

Prakash, S .

NATURE GENETICS, 2000, 25 (04) :458-461

[7] Roles of yeast DNA polymerases δ and ζ and of Rev1 in the bypass of abasic sites [J].

Haracska, L ;

Unk, I ;

Johnson, RE ;

Johansson, E ;

Burgers, PMJ ;

Prakash, S ;

Prakash, L .

GENES & DEVELOPMENT, 2001, 15 (08) :945-954

[8] Inefficient bypass of an abasic site by DNA polymerase η [J].

Haracska, L ;

Washington, MT ;

Prakash, S ;

Prakash, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (09) :6861-6866

[9] Replication past O6-methylguanine by yeast and human DNA polymerase η [J].

Haracska, L ;

Prakash, S ;

Prakash, L .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (21) :8001-8007

[10] Eukaryotic polymerases ι and ζ act sequentially to bypass DNA lesions [J].

Johnson, RE ;

Washington, MT ;

Haracska, L ;

Prakash, S ;

Prakash, L .

NATURE, 2000, 406 (6799) :1015-1019

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