Microextraction of nuclear proteins from single maize embryos

被引：21

作者：

Busk, PK ^{[1
]}

Pagès, M ^{[1
]}

机构：

[1] CSIC, Cid, Dept Mol Genet, ES-08034 Barcelona, Spain

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1997年 / 15卷 / 04期

关键词：

band shift assay; DNA binding protein; maize; nuclear protein extraction;

D O I：

10.1023/A:1007428802474

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of DNA binding proteins can be difficult when only small quantities of tissue expressing the desired protein are available. We present a protocol for the preparation of nuclear extracts from as little as 100 mg of tissue. This protocol is well suited for extraction of DNA binding proteins from tissues that are difficult to obtain in large quantities such as maize embryos.

引用

页码：371 / 376

页数：6

共 9 条

[1]

ABMAYR SM, 1993, CURRENT PROTOCOLS S, V11

[2]

BUSK K, 1997, IN PRESS PLANT CELL, V9

[3] CHARACTERIZATION OF A MAIZE G-BOX BINDING-FACTOR THAT IS INDUCED BY HYPOXIA [J].