Human cyclophilin has a significantly higher affinity for HIV-1 recombinant p55 than p24

被引：16

作者：

Bristow, R

Byrne, J

Squirell, J

Trencher, H

Carter, T

Rodgers, B

Saman, E

Duncan, J

机构：

[1] Murex Biotech Ltd, Dartford, Kent, England

[2] Innogent NV, B-9052 Ghent, Belgium

来源：

JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY | 1999年 / 20卷 / 04期

关键词：

HIV; gag; cyclophilin; p24; ELISA;

D O I：

10.1097/00042560-199904010-00002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The ability of cyclophilin to bind a panel of recombinant HIV-gag proteins was assessed using sensitive, quantitative, sandwich enzyme-linked immunosorbant assays (ELISAs). Significantly higher binding to cyclophilin was observed when recombinants contained at least 12 carboxy-terminal amino acids of p17 in addition to p24 sequences. These results indicate that the carboxy-terminus of p17 is important for optimal binding of cyclophilin to p24 and support the theory that cyclophilin acts on the uncleaved HIV-1 gag (p17-p24) precursor.

引用

页码：334 / 336

页数：3

共 8 条

[1] Cyclophilin A is required for the replication of group M human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus SIV(CPZ)GAB but not group O HIV-1 or other primate immunodeficiency viruses [J].