NK phenotypic markers and IL2 response in NK cells from elderly people

被引:187
作者
Borrego, F [1 ]
Alonso, MC [1 ]
Galiani, MD [1 ]
Carracedo, J [1 ]
Ramirez, R [1 ]
Ostos, B [1 ]
Peña, J [1 ]
Solana, R [1 ]
机构
[1] Hosp Univ Reina Sofia, Dept Immunol, E-14004 Cordoba, Spain
关键词
immunosenescence; aging; NK cells; CD69; proliferation; CD56; CD57;
D O I
10.1016/S0531-5565(98)00076-X
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
Immunosenescence is a process that primarily affects the T cell compartment of the immune system, although age-associated immunological alterations have: also been demonstrated in the NK cell phenotype and function. A significant expansion in the number of NK cells is found in aging. The NK cytotoxic capacity of total peripheral blood lymphocytes is also well preserved, not only in healthy elderly people but also in centenarians. However, NK cell killing of K562 is impaired when considered in a per-cell basis, and this defect is associated with defective signal transduction after activation more than a diminished conjugate formation or killing capacity. We have studied the phenotype of NK cells in elderly donors fulfilling the Senieur criteria. We have also studied the capacity of these cells to be activated by IL2 when different NR cell functions, other than cytotoxicity, are considered. Our results confirm the increased percentage of NK cells in the elderly due to the expansion of the CD56(dim) subset that also show an altered pattern of activation markers, whereas no differences were found in the CD56(britght) subset. The response of NK cells to IL2 was found to be impaired when proliferation, expression of CD69, and Ca2+ mobilization were considered, whereas TNF-alpha production was not significantly affected, These results suggest that human NK cells do not escape the aging process, although senescence have a differential effect on distinct NK cell biological functions, ranging from severe to negligible impairment, depending on the parameters considered. (C) 1999 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:253 / 265
页数:13
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