Detailed Mitochondrial Phenotyping by High Resolution Metabolomics

被引：37

作者：

Roede, James R. ^{[1
]}

Park, Youngja ^{[1
]}

Li, Shuzhao ^{[2
]}

Strobel, Frederick H. ^{[3
]}

Jones, Dean P. ^{[1
]}

机构：

[1] Emory Univ, Dept Med, Div Pulm Allergy & Crit Care Med, Atlanta, GA 30322 USA

[2] Emory Univ, Emory Vaccine Ctr, Yerkes Natl Primate Ctr, Atlanta, GA 30322 USA

[3] Emory Univ, Mass Spectrometry Ctr, Atlanta, GA 30322 USA

来源：

PLOS ONE | 2012年 / 7卷 / 03期

基金：

美国国家卫生研究院;

关键词：

GENDER-DIFFERENCES; METABOLISM; DISEASE; IDENTIFICATION; DYSFUNCTION;

D O I：

10.1371/journal.pone.0033020

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used "top-down'' metabolic profiling to determine the spectrum of metabolites present in liver mitochondria. High resolution mass spectral analyses and multivariate statistical tests provided global metabolic information about mitochondria and showed that liver mitochondria possess a significant phenotype based on gender and genotype. The data also show that mitochondria contain a large number of unidentified chemicals.

引用

页数：8

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