Genetically engineered wine yeast produces a high concentration of L-lactic acid of extremely high optical purity

被引：99

作者：

Saitoh, S

Ishida, N

Onishi, T

Tokuhiro, K

Nagamori, E

Kitamoto, K

Takahashi, H

机构：

[1] Toyota Cent Res & Dev Labs Inc, Biotechnol Lab, Aichi 4801192, Japan

[2] Toyota Motor Co, Toyota Biotechnol & Afforestn Lab, Aichi 4700201, Japan

[3] Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 05期

关键词：

D O I：

10.1128/AEM.71.5.2789-2792.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher.

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页码：2789 / 2792

页数：4

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