Direct pathogen detection from swab samples using a new high-throughput sequencing technology

被引：30

作者：

Yongfeng, H. ^{[1
]}

Fan, Y. ^{[1
]}

Jie, D. ^{[1
]}

Jian, Y. ^{[1
]}

Ting, Z. ^{[1
]}

Lilian, S. ^{[1
]}

Jin, Q. ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Inst Pathogen Biol, State Key Lab Mol Virol & Genet Engn, Beijing, Peoples R China

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2011年 / 17卷 / 02期

关键词：

Emerging infectious diseases; high-throughput sequencing technology; novel influenza A (H1N1) virus; Solexa system; viral detection;

D O I：

10.1111/j.1469-0691.2010.03246.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

P>The detection of emerging infectious diseases has been a continuing concern, especially with the novel influenza A (H1N1) viral pandemic of 2009. In the present study, we validated a 'second-generation' parallel sequencing platform for viral detection in swab samples collected during recent influenza virus infections in Beijing. This operation yielded millions of valid reads per sample and resulted in an almost complete spectrum of nucleotide information. Importantly, novel A (H1N1) and seasonal A (H3N2) influenza virus-derived sequences were detected without prior knowledge or use of genetic information in advance, suggesting that this approach could be a valuable tool for diagnosing emerging infectious diseases.

引用

页码：241 / 244

页数：4

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