Characterisation of polyhydroxyalkanoate copolymers with controllable four-monomer composition

被引:66
作者
Dai, Yu [1 ]
Lambert, Lynette [2 ]
Yuan, Zhiguo [1 ]
Keller, Jurg [1 ]
机构
[1] Univ Queensland, AWMC, Brisbane, Qld 4072, Australia
[2] Univ Queensland, CMR, Brisbane, Qld 4072, Australia
关键词
PHA; copolymer; GAOs; NMR; melting temperature; molecular weight;
D O I
10.1016/j.jbiotec.2008.01.013
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Polyhydroxyalkanoate (PHA) copolymers comprising the four monomers 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 3-hydroxy-2-methylvalerate (3HMV) and 3-hydroxy-2-methylbutyrate (3HMB) were generated using the recently discovered Defluviicoccus vanus-related glycogen accumulating organisms (DvGAOs) under anaerobic conditions without applying any nutrient limitations. The composition could be manipulated in a defined range by modifying the ratio of propionate and acetate provided in the feed stream. The PHAs produced were characterised as random copolymers (from propionate alone) or a mixture of random copolymers (from mixture of propionate and acetate) through microstructure analysis using C-13 NMR spectroscopy. The sequence distribution of all eight comonomer pairs in the carbonyl region of 3HB and 3HV was identified and assigned with confidence utilising two-dimensional heteronuclear multiple bond coherence (HMBC) spectroscopy. Weight average molecular weights were in the range 390-560 kg/mol. Differential scanning calorimetry (DSC) traces showed that the melting temperature (T-m) varied between 70 and 161 degrees C and glass transition temperature (T-g) ranged from -8 to 0 degrees C. The incorporation of considerable amounts of 3HMV and 3HMB monomer units introduced additional "defects" into the PHBV copolymer structure and hence greatly lowered the crystallinity. The data indicate the potential of these four-monomer PHAs to be employed for practical applications, considering their favourable properties and the cost-effective production process using a mixed culture and simple carbon sources. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:137 / 145
页数:9
相关论文
共 35 条
[1]  
ABE H, 2001, RIKEN REV, V42, P7
[2]   OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES [J].
ANDERSON, AJ ;
DAWES, EA .
MICROBIOLOGICAL REVIEWS, 1990, 54 (04) :450-472
[3]   STUDIES ON COPOLYESTER SYNTHESIS BY RHODOCOCCUS-RUBER AND FACTORS INFLUENCING THE MOLECULAR MASS OF POLYHYDROXYBUTYRATE ACCUMULATED BY METHYLOBACTERIUM-EXTORQUENS AND ALCALIGENES-EUTROPHUS [J].
ANDERSON, AJ ;
WILLIAMS, DR ;
TAIDI, B ;
DAWES, EA ;
EWING, DF .
FEMS MICROBIOLOGY LETTERS, 1992, 103 (2-4) :93-101
[4]   Blosynthesis and properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) polymers [J].
Asrar, J ;
Valentin, HE ;
Berger, PA ;
Tran, M ;
Padgette, SR ;
Garbow, JR .
BIOMACROMOLECULES, 2002, 3 (05) :1006-1012
[5]   Microbial synthesis of poly(3-hydroxyalkanoatels) by Pseudomonas aeruginosa from fatty acids:: Identification of higher monomer units and structural characterization [J].
Barbuzzi, T ;
Giuffrida, M ;
Impallomeni, G ;
Camazza, S ;
Ferreri, A ;
Guglielmino, SPP ;
Ballistreri, A .
BIOMACROMOLECULES, 2004, 5 (06) :2469-2478
[6]   SOME STRUCTURAL AND MECHANICAL-PROPERTIES OF BACTERIALLY PRODUCED POLY-BETA-HYDROXYBUTYRATE-CO-BETA-HYDROXYVALERATE [J].
BAUER, H ;
OWEN, AJ .
COLLOID AND POLYMER SCIENCE, 1988, 266 (03) :241-247
[7]   STEREOREGULARITY IN SYNTHETIC BETA-HYDROXYBUTYRATE AND BETA-HYDROXYVALERATE HOMOPOLYESTERS [J].
BLOEMBERGEN, S ;
HOLDEN, DA ;
BLUHM, TL ;
HAMER, GK ;
MARCHESSAULT, RH .
MACROMOLECULES, 1989, 22 (04) :1656-1663
[8]   ISODIMORPHISM IN BACTERIAL POLY(BETA-HYDROXYBUTYRATE-CO-BETA-HYDROXYVALERATE) [J].
BLUHM, TL ;
HAMER, GK ;
MARCHESSAULT, RH ;
FYFE, CA ;
VEREGIN, RP .
MACROMOLECULES, 1986, 19 (11) :2871-2876
[9]  
Braun S, 2000, 150 and More Basic NMR Experiments: A Practical Course Second Expanded Edition
[10]  
Chen GQ, 2000, PROG NAT SCI, V10, P843