Effect of nicotine on human umbilical vein endothelial cells (HUVECs) migration and angiogenesis

被引：35

作者：

Park, Yang Jin ^{[1
]}

Lee, Taeseung ^{[2
]}

Ha, Jongwon ^{[1
]}

Jung, In Mok ^{[3
]}

Chung, Jung Kee ^{[3
]}

Kim, Sang Joon ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Med, Dept Surg, Seoul 110744, South Korea

[2] Seoul Natl Univ Bundang Hosp, Dept Surg, Kyonggi Do, South Korea

[3] Seoul Metropolitan Borame Hosp, Dept Surg, Seoul, South Korea

来源：

VASCULAR PHARMACOLOGY | 2008年 / 49卷 / 01期

关键词：

HUVEC; endothelial cell; angiogenesis;

D O I：

10.1016/j.vph.2008.05.001

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effects of nicotine on vascular endothelial cells have not been completely elucidated. We performed this study to assess the changes in cellular behaviors of human umbilical vein endothelial cells (HUVECs) treated with nicotine. We examined changes in cell count and morphology and assayed cellular migration with Boyden chamber and microcapillary tube formation in a Matrigel matrix following treatment with various concentrations of nicotine. Compared to the control, nicotine stimulated cell proliferation, migration, and tube formation at concentrations similar to those found in smokers. Although there were no specific morphological changes in HUVECs treated with nicotine at the concentration similar to that in smokers, at high concentration (10(-4) M), morphological changes such as cytoplasmic vacuolization and irregular cell shape were observed, which were assumed to be the result of direct cytotoxicity of nicotine. In HUVECs, nicotine enhanced cellular proliferation, migration and angiogenesis in vitro, and thus caused a functional change, not a morphological change at a concentration similar to that in habitual smokers. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：32 / 36

页数：5

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