The type 2 capsule locus of Streptococcus pneumoniae

被引：87

作者：

Iannelli, F ^{[1
]}

Pearce, BJ ^{[1
]}

Pozzi, G ^{[1
]}

机构：

[1] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 08期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JB.181.8.2652-2654.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The type 2 capsule locus of Streptococcus pneumoniae was characterized in Avery's strain D39, which is the parent strain of the standard transformation recipients currently used in pneumococcal research and is largely used as a virulent strain in studies on the pathogenesis of pneumococcal infections, The capsule locus was sequenced by using a 21.7-kb PCR fragment from the D39 genome as a template. Sequence data analysis showed the presence of 18 open reading frames, 17 of which have the same direction of transcription and all of which are potentially involved in capsule biosynthesis. It was also shown that R36A and R6, which are unencapsulated (rough) derivatives of D39, carry a 7,504-bp deletion involving nine capsule genes.

引用

页码：2652 / 2654

页数：3

共 28 条

[1] 3 HIGHLY HOMOLOGOUS MEMBRANE-BOUND LIPOPROTEINS PARTICIPATE IN OLIGOPEPTIDE TRANSPORT BY THE AMI SYSTEM OF THE GRAM-POSITIVE STREPTOCOCCUS-PNEUMONIAE [J].

ALLOING, G ;

DEPHILIP, P ;

CLAVERYS, JP .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 241 (01) :44-58

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3] Sequence and transcriptional analysis of a DNA region involved in the production of capsular polysaccharide in Streptococcus pneumoniae type 3 [J].

Arrecubieta, C ;

Garcia, E ;

Lopez, R .

GENE, 1995, 167 (1-2) :1-7

[4]

Austrian R., 1984, BACTERIAL VACCINES, P257

[5] STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES INDUCTION OF TRANSFORMATION BY A DESOXYRIBONUCLEIC ACID FRACTION ISOLATED FROM PNEUMOCOCCUS TYPE III [J].

Avery, Oswald T. ;

MacLeod, Colin M. ;

McCarty, Maclyn .

JOURNAL OF EXPERIMENTAL MEDICINE, 1944, 79 (02) :137-158

[6] EFFECT OF DEFINED POINT MUTATIONS IN THE PNEUMOLYSIN GENE ON THE VIRULENCE OF STREPTOCOCCUS-PNEUMONIAE [J].

BERRY, AM ;

ALEXANDER, JE ;

MITCHELL, TJ ;

ANDREW, PW ;

HANSMAN, D ;

PATON, JC .

INFECTION AND IMMUNITY, 1995, 63 (05) :1969-1974

[7] CLONING OF STREPTOCOCCUS-PNEUMONIAE DNA FRAGMENTS IN ESCHERICHIA-COLI REQUIRES VECTORS PROTECTED BY STRONG TRANSCRIPTIONAL TERMINATORS [J].

CHEN, JD ;

MORRISON, DA .

GENE, 1987, 55 (2-3) :179-187

[8] Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae [J].

Coffey, TJ ;

Enright, MC ;

Daniels, M ;

Morona, JK ;

Morona, R ;

Hryniewicz, W ;

Paton, JC ;

Spratt, BG .

MOLECULAR MICROBIOLOGY, 1998, 27 (01) :73-83

[9] ANALYSIS OF STREPTOCOCCUS-PNEUMONIAE SEQUENCES CLONED INTO ESCHERICHIA-COLI - EFFECT OF PROMOTER STRENGTH AND TRANSCRIPTION TERMINATORS [J].

DILLARD, JP ;

YOTHER, J .

JOURNAL OF BACTERIOLOGY, 1991, 173 (16) :5105-5109

[10] CHARACTERIZATION OF THE CASSETTE CONTAINING GENES FOR TYPE-3 CAPSULAR POLYSACCHARIDE BIOSYNTHESIS IN STREPTOCOCCUS-PNEUMONIAE [J].

DILLARD, JP ;

VANDERSEA, MW ;

YOTHER, J .

JOURNAL OF EXPERIMENTAL MEDICINE, 1995, 181 (03) :973-983

← 1 2 3 →