Temporal and regional changes during focal ischemia in rat brain studied by proton spectroscopic imaging and quantitative diffusion NMR imaging

被引:44
作者
Dreher, W
Kühn, B
Gyngell, ML
Busch, E
Niendorf, T
Hossmann, KA
Leibfritz, D
机构
[1] Univ Bremen, Fachbereich Chem 2, D-28334 Bremen, Germany
[2] Max Planck Inst Neurol Forsch, D-50931 Koln, Germany
关键词
focal ischemia; rat brain; proton spectroscopic imaging; diffusion-weighted NMR imaging;
D O I
10.1002/mrm.1910390605
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
The early development of focal ischemia after permanent occlusion of the right middle cerebral artery (MCA) was studied in six rats using interleaved measurements by diffusion-weighted NMR imaging (DWI) of water and two variants of proton spectroscopic imaging (SI), multiecho SI (TE: 136, 272, 408 ms) and short TE SI (TE: 20 ms). Measurements on a 4.7-T NMR imaging system were performed between the control phase and approximately 6 h postocclusion. In the center of the ischemic lesion of all rats, the apparent diffusion coefficient (ADC) decreased rapidly to 84.4 +/- 4.2% (mean +/- SD) of the control values approximately 2 min postocclusion. Approximately 6 h postocclusion, the ADC was reduced to 67.1 +/- 5.9%. In contrast, large differences between the animals were observed for the temporal increase of lactate (Lac) in the ipsilateral hemisphere. The maximum Lac signal was reached in four rats after 0.5-1.5 h, and in two rats was not reached even after 6 h postocclusion. Six h postocclusion, SI spectra measured at a TE of 136 ms revealed a decrease in the CH3 signal of N-acetylaspartate (NAA) to 67 +/- 13% of the control values. Differences were observed between the spatial regions of decreased NAA and increased Lac. In the lesions, a T-2 relaxation time of Lac of 292 +/- 40 ms, considering a J-coupling constant of 6.9 Hz, was measured. Furthermore, a prolongation;of the T-2 of the CH3 signal of creatine/phosphocreatine (Cr/PCr) was observed in the lesion, from 163 +/- 22 ms during control to 211 +/- 41 ms approximately 6 h postocclusion. The experiments proved that DWI and proton SI are valuable tools to provide complementary information on processes associated with brain infarcts.
引用
收藏
页码:878 / 888
页数:11
相关论文
共 48 条
[1]   ABSORPTION-SPECTRA FROM PHASE-MODULATED SPIN ECHOES [J].
BAX, A ;
MEHLKOPF, AF ;
SMIDT, J .
JOURNAL OF MAGNETIC RESONANCE, 1979, 35 (03) :373-377
[2]   HIGH-RESOLUTION H-1 NUCLEAR MAGNETIC-RESONANCE STUDY OF CEREBRAL HYPOXIA INVIVO [J].
BEHAR, KL ;
DENHOLLANDER, JA ;
STROMSKI, ME ;
OGINO, T ;
SHULMAN, RG ;
PETROFF, OAC ;
PRICHARD, JW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (16) :4945-4948
[3]   ASSIGNMENT OF RESONANCES IN THE H-1 SPECTRUM OF RAT-BRAIN BY 2-DIMENSIONAL SHIFT CORRELATED AND J-RESOLVED NMR-SPECTROSCOPY [J].
BEHAR, KL ;
OGINO, T .
MAGNETIC RESONANCE IN MEDICINE, 1991, 17 (02) :285-303
[4]   MECHANISM OF DETECTION OF ACUTE CEREBRAL-ISCHEMIA IN RATS BY DIFFUSION-WEIGHTED MAGNETIC-RESONANCE MICROSCOPY [J].
BENVENISTE, H ;
HEDLUND, LW ;
JOHNSON, GA .
STROKE, 1992, 23 (05) :746-754
[5]   CEREBRAL METABOLISM IN MAN AFTER ACUTE STROKE - NEW OBSERVATIONS USING LOCALIZED PROTON NMR-SPECTROSCOPY [J].
BRUHN, H ;
FRAHM, J ;
GYNGELL, ML ;
MERBOLDT, KD ;
HANICKE, W ;
SAUTER, R .
MAGNETIC RESONANCE IN MEDICINE, 1989, 9 (01) :126-131
[6]   RAPID MONITORING OF CHANGES IN WATER DIFFUSION-COEFFICIENTS DURING REVERSIBLE ISCHEMIA IN CAT AND RAT-BRAIN [J].
DAVIS, D ;
ULATOWSKI, J ;
ELEFF, S ;
IZUTA, M ;
MORI, S ;
SHUNGU, D ;
VANZIJL, PCM .
MAGNETIC RESONANCE IN MEDICINE, 1994, 31 (04) :454-460
[7]   CORRELATION OF RAPID CHANGES IN THE AVERAGE WATER DIFFUSION CONSTANT AND THE CONCENTRATIONS OF LACTATE AND ATP BREAKDOWN PRODUCTS DURING GLOBAL-ISCHEMIA IN CAT BRAIN [J].
DECANNIERE, C ;
ELEFF, S ;
DAVIS, D ;
VANZIJL, PCM .
MAGNETIC RESONANCE IN MEDICINE, 1995, 34 (03) :343-352
[8]   PARAMETRIC MULTIECHO PROTON SPECTROSCOPIC IMAGING - APPLICATION TO THE RAT-BRAIN IN-VIVO [J].
DREHER, W ;
LEIDFRITZ, D .
MAGNETIC RESONANCE IMAGING, 1995, 13 (05) :753-761
[9]   CHANGES IN ORGANIC SOLUTES, VOLUME, ENERGY-STATE, AND METABOLISM ASSOCIATED WITH OSMOTIC-STRESS IN A GLIAL-CELL LINE - A MULTINUCLEAR NMR-STUDY [J].
FLOGEL, U ;
NIENDORF, T ;
SERKOWA, N ;
BRAND, A ;
HENKE, J ;
LEIBFRITZ, D .
NEUROCHEMICAL RESEARCH, 1995, 20 (07) :793-802
[10]   DUAL INTERLEAVED H-1 AND PROTON-DECOUPLED-P-31 IN-VIVO CHEMICAL-SHIFT IMAGING OF HUMAN BRAIN [J].
GONEN, O ;
HU, J ;
MURPHYBOESCH, J ;
STOYANOVA, R ;
BROWN, TR .
MAGNETIC RESONANCE IN MEDICINE, 1994, 32 (01) :104-109