Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells

被引：56

作者：

Millard, AC

Jin, L

Lewis, A

Loew, LM

机构：

[1] Univ Connecticut, Ctr Hlth, Ctr Biomed Imaging Technol, Farmington, CT 06030 USA

[2] Hebrew Univ Jerusalem, Div Appl Phys, IL-91904 Jerusalem, Israel

来源：

OPTICS LETTERS | 2003年 / 28卷 / 14期

关键词：

D O I：

10.1364/OL.28.001221

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report what. is to our knowledge the first optical imaging of voltage-clamped cells by second-harmonic generation. For the membrane-staining styryl dye di-4-ANEPPS, we determined the sensitivity of second-harmonic generation to be 18%/100 mV at an excitation wavelength of 850 nm. This sensitivity is significantly better than the optimal 10%/100 mV under fluorescence and further establishes the importance of second-harmonic generation for the functional imaging of membrane potential in living cells. (C) 2003 Optical Society of America.

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页码：1221 / 1223

页数：3

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