Trans-SILAC: sorting out the non-cell-autonomous proteome

被引：26

作者：

Rechavi, Oded ^{[1
]}

Kalman, Matan ^{[2
]}

Fang, Yuan ^{[3
]}

Vernitsky, Helly ^{[1
,4
,5
]}

Jacob-Hirsch, Jasmine ^{[4
,5
]}

Foster, Leonard J. ^{[3
]}

Kloog, Yoel ^{[1
]}

Goldstein, Itamar ^{[4
,5
]}

机构：

[1] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Neurobiol, IL-69978 Tel Aviv, Israel

[2] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Biochem, IL-69978 Tel Aviv, Israel

[3] Univ British Columbia, Dept Biochem & Mol Biol, Ctr High Throughput Biol, Vancouver, BC V5Z 1M9, Canada

[4] Chaim Sheba Med Ctr, Canc Res Ctr, IL-52621 Tel Hashomer, Israel

[5] Tel Aviv Univ, Sackler Sch Med, IL-69978 Tel Aviv, Israel

来源：

NATURE METHODS | 2010年 / 7卷 / 11期

基金：

加拿大健康研究院;

关键词：

INTERCELLULAR TRANSFER; NK CELLS; T-CELL; TROGOCYTOSIS; PROTEINS; ACQUISITION; CAPTURE; NKG2D;

D O I：

10.1038/nmeth.1513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-cell-autonomous proteins are incorporated into cells that form tight contacts or are invaded by bacteria, but identifying the full repertoire of transferred proteins has been a challenge. Here we introduce a quantitative proteomics approach to sort out non-cell-autonomous proteins synthesized by other cells or intracellular pathogens. Our approach combines stable-isotope labeling of amino acids in cell culture (SILAC), high-purity cell sorting and bioinformatics analysis to identify the repertoire of relevant non-cell-autonomous proteins. This `trans-SILAC' method allowed us to discover many proteins transferred from human B to natural killer cells and to measure biosynthesis rates of Salmonella enterica proteins in infected human cells. Trans-SILAC should be a useful method to examine protein exchange between different cells of multicellular organisms or pathogen and host.

引用

页码：923 / U84

页数：9

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