Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR -: art. no. e134

被引：43

作者：

Hodson, JA ^{[1
]}

Bailis, JM ^{[1
]}

Forsburg, SL ^{[1
]}

机构：

[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 21期

关键词：

D O I：

10.1093/nar/gng134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently incorporates H-3-thymidine into nuclear DNA as monitored by scintillation counting. These strains also incorporate the thymidine analog Bromodeoxy uridine (BUdR) into newly replicated DNA, which can be detected by immunofluorescence and flow cytometry. This strain provides a valuable tool for direct study of DNA replication in S.pombe.

引用

页数：8

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