Expressed sequences from the basidiomycetous tree pathogen Heterobasidion annosum during early infection of scots pine

被引:43
作者
Karlsson, M [1 ]
Olson, Å [1 ]
Stenlid, J [1 ]
机构
[1] Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, SE-75007 Uppsala, Sweden
关键词
basidiomycete; Heterobasidion annosum; annosum root rot; tree pathogen; forest disease; cDNA sequencing; expressed sequence tags;
D O I
10.1016/S1087-1845(02)00586-8
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The pattern of gene expression of the basidiomycete Heterobasidion annosum, causal agent of the root rot of conifers, was analysed during its interaction with pine roots. A complementary DNA (cDNA) library was constructed from total RNA extracted from H. annosum mycelia challenged with Scots pine seedling roots for 6 and 72 h. Single pass sequencing of 1148 randomly selected cDNA clones resulted in 923 expressed sequence tags (ESTs). Contig analysis and sequence comparisons identified 318 unigene sequences, of which 62 were repeatedly sampled. A putative cellular function was assigned to 223 contigs (70%) that showed a moderate to high homology to protein sequences from public databases. Variations in expression levels during the infection process were monitored on a set of 96 unigenes by reverse northern using dot hybridisation. Seven unigenes (7%) were shown to be either up (4) or down (3) regulated during interaction of the fungus with pine roots. Fungal genes differentially expressed during contact with roots include genes encoding mitochondrial proteins, a cytochrome P450 and a vacuolar ATP synthase. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:51 / 59
页数:9
相关论文
共 46 条
  • [1] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT
    ADAMS, MD
    KELLEY, JM
    GOCAYNE, JD
    DUBNICK, M
    POLYMEROPOULOS, MH
    XIAO, H
    MERRIL, CR
    WU, A
    OLDE, B
    MORENO, RF
    KERLAVAGE, AR
    MCCOMBIE, WR
    VENTER, JC
    [J]. SCIENCE, 1991, 252 (5013) : 1651 - 1656
  • [2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [3] STUDIES ON THE INFECTION OF NORWAY SPRUCE ROOTS BY HETEROBASIDION-ANNOSUM
    ASIEGBU, F
    DANIEL, G
    JOHANSSON, M
    [J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1993, 71 (12): : 1552 - 1561
  • [4] DEFENSE-RELATED REACTIONS OF SEEDLING ROOTS OF NORWAY SPRUCE TO INFECTION BY HETEROBASIDION-ANNOSUM (FR.) BREF
    ASIEGBU, FO
    DANIEL, G
    JOHANSSON, M
    [J]. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1994, 45 (01) : 1 - 19
  • [5] Asiegbu FO, 1998, HETEROBASIDION ANNOSUM: BIOLOGY, ECOLOGY, IMPACT AND CONTROL, P167
  • [6] ASIEGBU FO, 2000, P NORD BALT M FOR PA, P9
  • [7] THE VACUOLAR ATPASE OF NEUROSPORA-CRASSA
    BOWMAN, BJ
    VAZQUEZLASLOP, N
    BOWMAN, EJ
    [J]. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1992, 24 (04) : 361 - 370
  • [8] CAPRETTI P, 1990, EUR J FOREST PATHOL, V20, P231
  • [9] CHASE TE, 1985, THESIS U VERMONT BUR
  • [10] Base-calling of automated sequencer traces using phred.: II.: Error probabilities
    Ewing, B
    Green, P
    [J]. GENOME RESEARCH, 1998, 8 (03): : 186 - 194