Different kinases phosphorylate nucleophosmin/B23 at different sites during G2 and M phases of the cell cycle

被引：31

作者：

Jiang, PS ^{[1
]}

Chang, JH ^{[1
]}

Yung, BYM ^{[1
]}

机构：

[1] Chang Gung Univ, Coll Med, Dept Pharmacol, Canc Biochem Lab, Tao Yuan 333, Taiwan

来源：

CANCER LETTERS | 2000年 / 153卷 / 1-2期

关键词：

nucleophosmin/B23; phosphorylation; cell cycle;

D O I：

10.1016/S0304-3835(00)00362-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The recombinant GST-nucleophosmin/B23 and the truncated mutants were tested for phosphorylation in cell-fret extracts of G(2) and M phases or by purified kinases. Our results indicated that a threonine residue at amino acids (a.a.) 185-240 was phosphorylated by cdc2 kinase during the entry of mitosis while the serine phosphorylation site at the middle acidic portion of the molecule (a.a. 83-152) was phosphorylated by casein kinase Ii during G(2) phase. Our results also showed that there was possibly another serine phosphorylation at site other than the middle portion of nucleophosmin/B23 (a.a. 83-152) during the entry of cells into mitosis. The demonstration of the characteristic changes in phosphorylation of nucleophosmin/B23 during the cell cycle implicates important role of nucleophosmin/B23 in the control of the fate of nucleoli and cell growth. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：151 / 160

页数：10

共 43 条

[1]

ADACHI Y, 1993, J BIOL CHEM, V268, P13930

[2] MAJOR NUCLEOLAR PROTEINS SHUTTLE BETWEEN NUCLEUS AND CYTOPLASM [J].

BORER, RA ;

LEHNER, CF ;

EPPENBERGER, HM ;

NIGG, EA .

CELL, 1989, 56 (03) :379-390

[3] CYCLIN-LIKE ACCUMULATION AND LOSS OF THE PUTATIVE KINETOCHORE MOTOR CENP-E RESULTS FROM COUPLING CONTINUOUS SYNTHESIS WITH SPECIFIC DEGRADATION AT THE END OF MITOSIS [J].

BROWN, KD ;

COULSON, RMR ;

YEN, TJ ;

CLEVELAND, DW .

JOURNAL OF CELL BIOLOGY, 1994, 125 (06) :1303-1312

[4]

CHAN PK, 1989, CANCER RES, V49, P3271

[5]

CHAN PK, 1986, J BIOL CHEM, V261, P14335

[6] THE MAJOR PHOSPHORYLATION SITE OF NUCLEOPHOSMIN (B23) IS PHOSPHORYLATED BY A NUCLEAR KINASE-II [J].

CHAN, PK ;

LIU, QR ;

DURBAN, E .

BIOCHEMICAL JOURNAL, 1990, 270 (02) :549-552

[7]

CHAN PK, 1986, J BIOL CHEM, V261, P1868

[8] CHARACTERIZATION OF THE CDNA-ENCODING HUMAN NUCLEOPHOSMIN AND STUDIES OF ITS ROLE IN NORMAL AND ABNORMAL GROWTH [J].

CHAN, WY ;

LIU, QR ;

BORJIGIN, J ;

BUSCH, H ;

RENNERT, OM ;

TEASE, LA ;

CHAN, PK .

BIOCHEMISTRY, 1989, 28 (03) :1033-1039

[9]

CHANG JH, 1988, J BIOL CHEM, V263, P12824

[10] CELL-CYCLE PHASE-DEPENDENT CHANGES OF LOCALIZATION AND OLIGOMERIZATION STATES OF NUCLEOPHOSMIN/B23 [J].

CHOU, YH ;

YUNG, BYM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 217 (01) :313-325

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