The use of suppression subtractive hybridization for the study of SDF-1α induced gene-expression in human endothelial cells

被引：6

作者：

Neuhaus, T

Lutz, C

Stier, S

Totzke, G

Gruenewald, E

Fronhoffs, S

Sachinidis, A

Vetter, H

Ko, YD

机构：

[1] Univ Bonn, Med Poliklin, D-53111 Bonn, Germany

[2] Univ Dusseldorf, Inst Mol Med, D-40225 Dusseldorf, Germany

[3] Ctr Physiol & Pathophysiol, D-50931 Cologne, Germany

来源：

MOLECULAR AND CELLULAR PROBES | 2003年 / 17卷 / 05期

关键词：

stromal cell-derived factor; human endothelial cells; suppression subtractive hybridization;

D O I：

10.1016/j.mcp.2003.07.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Stromal cell-derived factor-1 (SDF-1), the only ligand of the CXCR4 receptor, is mainly known as a chemotactic factor for hematopoietic progenitor cells. However, studies of knock-out mice have shown malformation of different organ-systems suggesting that SDF-1 may have a role in angiogenesis and cardiac and cerebral development. However, the underlying mechanisms of its action are largely unknown. Therefore, we performed suppression subtractive hybridization (SSH) in order to identify genes that are differentially expressed after stimulation of human arterial endothelial cells (HUAEC) with SDF-1. Using SSH we found ten genes, with varied functions, whose mRNA expression is induced by SDF-1alpha in HUAEC. We show that SSH is a reliable method for identifying differentially expressed genes and that SDF-1alpha may have more functions than previously reported. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：245 / 252

页数：8

共 61 条

[1]

Adams S. M., 1992, Human Molecular Genetics, V1, P91, DOI 10.1093/hmg/1.2.91

[2] The chemokine SDF-1 is a chemoattractant for human CD34(+) hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34(+) progenitors to peripheral blood [J].