The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

被引：132

作者：

Cheng, Hongtao ^{[1
]}

Liu, Hongbo ^{[1
]}

Deng, Yong ^{[1
]}

Xiao, Jinghua ^{[1
]}

Li, Xianghua ^{[1
]}

Wang, Shiping ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

来源：

PLANT PHYSIOLOGY | 2015年 / 167卷 / 03期

基金：

中国国家自然科学基金;

关键词：

DISEASE RESISTANCE; BACTERIAL-BLIGHT; MULTIPLE PHYTOHORMONES; CONFERRING RESISTANCE; BASAL RESISTANCE; OPPOSITE ROLES; PLANT DEFENSE; GENE; ARABIDOPSIS; EXPRESSION;

D O I：

10.1104/pp.114.256016

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Blast caused by fungal Magnaporthe oryzae is a devastating disease of rice (Oryza sativa) worldwide, and this fungus also infects barley (Hordeum vulgare). At least 11 rice WRKY transcription factors have been reported to regulate rice response to Magnaporthe oryzae either positively or negatively. However, the relationships of these WRKYs in the rice defense signaling pathway against Magnaporthe oryzae are unknown. Previous studies have revealed that rice WRKY13 (as a transcriptional repressor) and WRKY45-2 enhance resistance to Magnaporthe oryzae. Here, we show that rice WRKY42, functioning as a transcriptional repressor, suppresses resistance to Magnaporthe oryzae. WRKY42-RNA interference (RNAi) and WRKY42-overexpressing (oe) plants showed increased resistance and susceptibility to Magnaporthe oryzae, accompanied by increased or reduced jasmonic acid (JA) content, respectively, compared with wild-type plants. JA pretreatment enhanced the resistance of WRKY42-oe plants to Magnaporthe oryzae. WRKY13 directly suppressed WRKY42. WRKY45-2, functioning as a transcriptional activator, directly activated WRKY13. In addition, WRKY13 directly suppressed WRKY45-2 by feedback regulation. The WRKY13-RNAi WRKY45-2-oe and WRKY13-oe WRKY42-oe double transgenic lines showed increased susceptibility to Magnaporthe oryzae compared with WRKY45-2-oe and WRKY13-oe plants, respectively. These results suggest that the three WRKYs form a sequential transcriptional regulatory cascade. WRKY42 may negatively regulate rice response to Magnaporthe oryzae by suppressing JA signaling-related genes, and WRKY45-2 transcriptionally activates WRKY13, whose encoding protein in turn transcriptionally suppresses WRKY42 to regulate rice resistance to Magnaporthe oryzae.

引用

页码：1087 / +

页数：29

共 62 条

[1]

[Anonymous], 2013, Genetics and genomics of rice. Plant genetics and genomics: crops and models, DOI DOI 10.1007/978-1-4614-7903-1_11

[2] Comparative Transcriptome Profiling of the Early Response to Magnaporthe oryzae in Durable Resistant vs Susceptible Rice (Oryza sativa L.) Genotypes [J].

Bagnaresi, Paolo ;

Biselli, Chiara ;

Orru, Luigi ;

Urso, Simona ;

Crispino, Laura ;

Abbruscato, Pamela ;

Piffanelli, Pietro ;

Lupotto, Elisabetta ;

Cattivelli, Luigi ;

Vale, Giampiero .

PLOS ONE, 2012, 7 (12)

[3] Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKY13, a gene regulating rice disease resistance [J].

Cai, Meng ;

Qiu, Deyun ;

Yuan, Ting ;

Ding, Xinhua ;

Li, Hongjing ;

Duan, Liu ;

Xu, Caiguo ;

Li, Xianghua ;

Wang, Shiping .

PLANT CELL AND ENVIRONMENT, 2008, 31 (01) :86-96

[4] Expression pattern of a rice disease resistance gene Xa3/Xa26 is differentially regulated by the genetic backgrounds and developmental stages that influence its function [J].

Cao, Yinglong ;

Ding, Xinhua ;

Cai, Meng ;

Zhao, Jing ;

Lin, Yongjun ;

Li, Xianghua ;

Xu, Caiguo ;

Wang, Shiping .

GENETICS, 2007, 177 (01) :523-533

[5] Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor [J].

Chen, CH ;

Chen, ZX .

PLANT PHYSIOLOGY, 2002, 129 (02) :706-716

[6] Arabidopsis Phytochrome A Directly Targets Numerous Promoters for Individualized Modulation of Genes in a Wide Range of Pathways [J].

Chen, Fang ;

Li, Bosheng ;

Li, Gang ;

Charron, Jean-Benoit ;

Dai, Mingqiu ;

Shi, Xiarong ;

Deng, Xing Wang .

PLANT CELL, 2014, 26 (05) :1949-1966

[7] Comparative analyses of genomic locations and race specificities of loci for quantitative resistance to Pyricularia grisea in rice and barley [J].

Chen, H ;

Wang, SP ;

Xing, YZ ;

Xu, CG ;

Hayes, PM ;

Zhang, QF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2544-2549

[8] New gene for bacterial blight resistance in rice located on chromosome 12 identified from Minghui 63, an elite restorer line [J].

Chen, HL ;

Wang, SP ;

Zhang, QF .

PHYTOPATHOLOGY, 2002, 92 (07) :750-754

[9]

[成洪涛 Cheng HongTao], 2014, [中国科学. 生命科学, Scientia Sinica Vitae], V44, P784

[10] Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice [J].

Chuio, Tetsuya ;

Takai, Ryota ;

Akimoto-Tomiyama, Chiharu ;

Ando, Sugihiro ;

Minami, Eiichi ;

Nagamura, Yoshiaki ;

Kaku, Hanae ;

Shibuya, Naoto ;

Yasuda, Michiko ;

Nakashita, Hideo ;

Umemura, Kenji ;

Okada, Atsushi ;

Okada, Kazunori ;

Nojiri, Hideaki ;

Yamane, Hisakazu .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2007, 1769 (7-8) :497-505

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