共 61 条
Modulating the activity of the peptidyl transferase center of the ribosome
被引:19
作者:

论文数: 引用数:
h-index:
机构:
机构:
[1] Ctr Gen Regulat, Barcelona 08003, Spain
[2] Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany
来源:
关键词:
ribosome;
peptidyl transferase center;
peptide bond formation;
peptide release;
SecM;
TnaC;
D O I:
10.1261/rna.980308
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The peptidyl transferase (PT) center of the ribosome catalyzes two nucleophilic reactions, peptide bond formation between aminoacylated tRNA substrates and, together with release factor, peptide release. Structure and function of the PT center are modulated by binding of aminoacyl-tRNA or release factor, thus providing the basis for the specificity of catalysis. Another way by which the function of the PT center is controlled is signaling from the peptide exit tunnel. The SecM nascent peptide induces ribosome stalling, presumably by inhibition of peptide bond formation. Similarly, the release factor-induced hydrolytic activity of the PT center can be suppressed by the TnaC nascent peptide contained in the exit tunnel. Thus, local and long-range conformational rearrangements can lead to changes in the reaction specificity and catalytic activity of the PT center.
引用
收藏
页码:795 / 801
页数:7
相关论文
共 61 条
[1]
The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
[J].
Ban, N
;
Nissen, P
;
Hansen, J
;
Moore, PB
;
Steitz, TA
.
SCIENCE,
2000, 289 (5481)
:905-920

Ban, N
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Nissen, P
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Hansen, J
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Moore, PB
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Steitz, TA
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[2]
Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression
[J].
Bashan, A
;
Agmon, I
;
Zarivach, R
;
Schluenzen, F
;
Harms, J
;
Berisio, R
;
Bartels, H
;
Franceschi, F
;
Auerbach, T
;
Hansen, HAS
;
Kossoy, E
;
Kessler, M
;
Yonath, A
.
MOLECULAR CELL,
2003, 11 (01)
:91-102

论文数: 引用数:
h-index:
机构:

Agmon, I
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

论文数: 引用数:
h-index:
机构:

Schluenzen, F
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Harms, J
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Berisio, R
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Bartels, H
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Franceschi, F
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Auerbach, T
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Hansen, HAS
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Kossoy, E
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Kessler, M
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

Yonath, A
论文数: 0 引用数: 0
h-index: 0
机构:
Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel
[3]
The A2453-C2499 wobble base pair in Escherichia coli 23S ribosomal RNA is responsible for pH sensitivity of the peptidyltransferase active site conformation
[J].
Bayfield, MA
;
Thompson, J
;
Dahlberg, AE
.
NUCLEIC ACIDS RESEARCH,
2004, 32 (18)
:5512-5518

Bayfield, MA
论文数: 0 引用数: 0
h-index: 0
机构:
Brown Univ, Dept Mol Biol Cellular Biol & Biochem, Providence, RI 02912 USA Brown Univ, Dept Mol Biol Cellular Biol & Biochem, Providence, RI 02912 USA

Thompson, J
论文数: 0 引用数: 0
h-index: 0
机构:
Brown Univ, Dept Mol Biol Cellular Biol & Biochem, Providence, RI 02912 USA Brown Univ, Dept Mol Biol Cellular Biol & Biochem, Providence, RI 02912 USA

Dahlberg, AE
论文数: 0 引用数: 0
h-index: 0
机构:
Brown Univ, Dept Mol Biol Cellular Biol & Biochem, Providence, RI 02912 USA Brown Univ, Dept Mol Biol Cellular Biol & Biochem, Providence, RI 02912 USA
[4]
A conformational change in the ribosomal peptidyl transferase center upon active/inactive transition
[J].
Bayfield, MA
;
Dahlberg, AE
;
Schulmeister, U
;
Dorner, S
;
Barta, A
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2001, 98 (18)
:10096-10101

Bayfield, MA
论文数: 0 引用数: 0
h-index: 0
机构: Vienna Bioctr, Div Biochem, Inst Med Biochem, A-1030 Vienna, Austria

Dahlberg, AE
论文数: 0 引用数: 0
h-index: 0
机构: Vienna Bioctr, Div Biochem, Inst Med Biochem, A-1030 Vienna, Austria

Schulmeister, U
论文数: 0 引用数: 0
h-index: 0
机构: Vienna Bioctr, Div Biochem, Inst Med Biochem, A-1030 Vienna, Austria

Dorner, S
论文数: 0 引用数: 0
h-index: 0
机构: Vienna Bioctr, Div Biochem, Inst Med Biochem, A-1030 Vienna, Austria

Barta, A
论文数: 0 引用数: 0
h-index: 0
机构: Vienna Bioctr, Div Biochem, Inst Med Biochem, A-1030 Vienna, Austria
[5]
Essential mechanisms in the catalysis of peptide bond formation on the ribosome
[J].
Beringer, M
;
Bruell, C
;
Xiong, LQ
;
Pfister, P
;
Bieling, P
;
Katunin, VI
;
Mankin, AS
;
Böttger, EC
;
Rodnina, MV
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2005, 280 (43)
:36065-36072

论文数: 引用数:
h-index:
机构:

Bruell, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Xiong, LQ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Pfister, P
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

论文数: 引用数:
h-index:
机构:

Katunin, VI
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Mankin, AS
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Böttger, EC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Rodnina, MV
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany
[6]
The G2447A mutation does not affect ionization of a ribosomal group taking part in peptide bond formation
[J].
Beringer, M
;
Adio, S
;
Wintermeyer, W
;
Rodnina, M
.
RNA,
2003, 9 (08)
:919-922

论文数: 引用数:
h-index:
机构:

Adio, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Wintermeyer, W
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Rodnina, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany
[7]
Importance of tRNA interactions with 23S rRNA for peptide bond formation on the ribosome: studies with substrate analogs
[J].
Beringer, Malte
;
Rodnina, Marina V.
.
BIOLOGICAL CHEMISTRY,
2007, 388 (07)
:687-691

论文数: 引用数:
h-index:
机构:

Rodnina, Marina V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany
[8]
The ribosomal peptidyl transferase
[J].
Beringer, Malte
;
Rodnina, Marina V.
.
MOLECULAR CELL,
2007, 26 (03)
:311-321

论文数: 引用数:
h-index:
机构:

Rodnina, Marina V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany
[9]
Peptide bond formation does not involve acid-base catalysis by ribosomal residues
[J].
Bieling, P
;
Beringer, M
;
Adio, S
;
Rodnina, MV
.
NATURE STRUCTURAL & MOLECULAR BIOLOGY,
2006, 13 (05)
:423-428

论文数: 引用数:
h-index:
机构:

论文数: 引用数:
h-index:
机构:

Adio, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany

Rodnina, MV
论文数: 0 引用数: 0
h-index: 0
机构: Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany
[10]
The interaction between C75 of tRNA and the A loop of the ribosome stimulates peptidyl transferase activity
[J].
Brunelle, JL
;
Youngman, EM
;
Sharma, D
;
Green, R
.
RNA,
2006, 12 (01)
:33-39

Brunelle, JL
论文数: 0 引用数: 0
h-index: 0
机构:
Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA

Youngman, EM
论文数: 0 引用数: 0
h-index: 0
机构:
Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA

Sharma, D
论文数: 0 引用数: 0
h-index: 0
机构:
Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA

Green, R
论文数: 0 引用数: 0
h-index: 0
机构:
Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA