The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs

被引：296

作者：

Okamura, Katsutomo ^{[1
]}

Chung, Wei-Jen ^{[1
]}

Ruby, J. Graham ^{[2
,3
,4
]}

Guo, Huili ^{[2
,3
,4
]}

Bartel, David P. ^{[2
,3
,4
]}

Lai, Eric C. ^{[1
]}

机构：

[1] Sloan Kettering Inst, Dept Dev Biol, Rockefeller Res Labs 521, New York, NY 10065 USA

[2] MIT, Howard Hughes Med Inst, Cambridge, MA 02142 USA

[3] MIT, Dept Biol, Cambridge, MA 02142 USA

[4] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

来源：

NATURE | 2008年 / 453卷 / 7196期

关键词：

D O I：

10.1038/nature07015

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In contrast to microRNAs and Piwi- associated RNAs, short interfering RNAs ( siRNAs) are seemingly dispensable for host- directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA- generating enzyme Dicer- 2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology(1,2). Moreover, endogenous Drosophila siRNAs have not yet been identified. Here we report that siRNAs derived from long hairpin RNA genes ( hpRNAs) programme Slicer complexes that can repress endogenous target transcripts. The Drosophila hpRNA pathway is a hybrid mechanism that combines canonical RNA interference factors ( Dicer- 2, Hen1 ( known as CG12367) and Argonaute 2) with a canonical microRNA factor ( Loquacious) to generate similar to 21- nucleotide siRNAs. These novel regulatory RNAs reveal unexpected complexity in the sorting of small RNAs, and open a window onto the biological usage of endogenous RNA interference in Drosophila.

引用

页码：803 / U8

页数：5

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