A molecular switch for translational control in taste memory consolidation

被引:70
作者
Belelovsky, K
Elkobi, A
Kaphzan, H
Nairn, AC
Rosenblum, K [1 ]
机构
[1] Univ Haifa, Fac Sci, Dept Neurobiol & Ethol, Ctr Brain & Behav, IL-30905 Haifa, Israel
[2] Yale Univ, Dept Psychiat, New Haven, CT 06520 USA
关键词
eEF2; ERK1/2; long-term memory; rat; translation regulation;
D O I
10.1111/j.1460-9568.2005.04428.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In a variety of species memory consolidation following different learning paradigms has been shown to be dependent on protein synthesis. However, it is not known whether modulation of protein synthesis is a critical component of the consolidation process, nor is the identity of any protein(s) subject to translational regulation, known. We report here that phosphorylation of eukaryotic elongation factor-2 (eEF2), an indicator for translational elongation attenuation, is correlated with input that produces taste memory consolidation in the relevant cortex of rat. The temporal pattern of eEF2 phosphorylation is similar to extra-cellular regulated kinase 2 (ERK2) activation and S6K1 phosphorylation, which are known to stimulate translation initiation. In addition, increased eEF2 phosphorylation and increased alpha CaMKII expression is detected in a synaptoneurosomal fraction made from taste cortex following memory consolidation. These results suggest that increased initiation rate together with decreased elongation rate, during memory consolidation, shift the rate-limiting step of protein synthesis, to produce a local switch-like effect in the expression of neuronal proteins.
引用
收藏
页码:2560 / 2568
页数:9
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