Expression of OsDREB2A transcription factor confers enhanced dehydration and salt stress tolerance in rice (Oryza sativa L.)

被引：128

作者：

Mallikarjuna, Garladinne ^{[1
,2
]}

Mallikarjuna, Kokkanti ^{[2
]}

Reddy, M. K. ^{[1
]}

Kaul, Tanushri ^{[1
]}

机构：

[1] ICGEB, Plant Mol Biol Lab, New Delhi 110067, India

[2] Acharya Nagarjuna Univ, Dept Bot & Microbiol, Hyderabad 522510, Andhra Pradesh, India

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 08期

关键词：

Dehydration-responsive element binding transcription factors; Drought; Rice; Salinity; Transgenic; RESPONSIVE GENE-EXPRESSION; SIGNAL-TRANSDUCTION; FUNCTIONAL-ANALYSIS; LOW-TEMPERATURE; DROUGHT; ARABIDOPSIS; PATHWAYS; WHEAT; DREB2;

D O I：

10.1007/s10529-011-0620-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Stress responsive transcriptional regulation is an adaptive strategy of plants that alleviates the adverse effects of environmental stresses. The ectopic overexpression of Dehydration-Responsive Element Binding transcription factors (DREBs) either in homologous or in heterologous plants improved stress tolerance indicating the DRE/DREB regulon is conserved across plants. We developed 30 transgenic T(0) rice plants overexpressing OsDREB2A which were devoid of any growth penalty or phenotypic abnormalities during stressed or non-stressed conditions. Integration of T-DNA in the rice genome and stress inducible overexpression of OsDREB2A had occurred in these transgenic lines. Functional analyses of T(1)-3 and T(1)-10 lines revealed significant tolerance to osmotic, salt and dehydration stresses during simulated stress conditions with enhanced growth performance as compared to wild type. OsDREB2A, thus, confers stress tolerance in homologous rice system that failed in the heterologous Arabidopsis system earlier.

引用

页码：1689 / 1697

页数：9

共 16 条

[1] Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and its phosphorylation negatively regulates its DNA-binding activity [J].