Flagellin promotes the proliferation of gastric cancer cells via the Toll-like receptor 5

被引:81
作者
Song, Eun-Jung [1 ]
Kang, Min-Jung [1 ]
Kim, Yong-Seok [2 ]
Kim, Sun-Moon [2 ]
Lee, Sang-Eok [3 ]
Kim, Chang-Hwan [4 ]
Kim, Dong-Jae [5 ]
Park, Jong-Hwan [1 ]
机构
[1] Konyang Univ, Coll Med, Dept Biochem, Taejon 302711, South Korea
[2] Konyang Univ, Coll Med, Dept Internal Med, Taejon 302711, South Korea
[3] Konyang Univ, Coll Med, Dept Surg, Taejon 302711, South Korea
[4] Agcy Def Dev, Taejon 305152, South Korea
[5] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA
基金
新加坡国家研究基金会;
关键词
flagellin; gastric cancer; proliferation; Toll-like receptor; extracellular-signal regulated kinase; NF-KAPPA-B; HELICOBACTER-PYLORI; SIGNALING PATHWAYS; EPITHELIAL-CELLS; TUMOR-GROWTH; INVASION; CARCINOMA; SURVIVAL; AGONISTS; TLR4;
D O I
10.3892/ijmm.2011.656
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
100103 [病原生物学]; 100218 [急诊医学];
摘要
Signaling of the Toll-like receptor (TLR) is closely associated with tumor development and progression processes including cell proliferation, angiogenesis, metastasis, and immunosuppression. In this study, we examined the expression of TLR5 in gastric cancer cells and its function in cell proliferation. RT-PCR revealed that the TLR5 gene was expressed in all gastric cancer cell lines examined, SNU638, SNU601, SNU216, and AGS. The TLR5 agonist, flagellin, induced IL-8 production and NF-kappa B activation in the gastric cancer cell lines. In addition, flagellin enhanced the proliferation of all gastric cancer cells examined, whereas LPS did not affect that of SNU638 cells. Blockade of TLR5 using an antibody, restored the proliferation of SNU638 cells enhanced by flagellin, indicating that TLR5 is essential for cell proliferation by flagellin. Flagellin also led to phosphorylation of ERK in SNU638 cells. The ERK inhibitor, PD98059, restored the proliferation ability of SNU638 cells enhanced by flagellin, suggesting that ERK may play an important role in the proliferation of gastric cancer cells. These findings suggest that TLR5 may play an important role in tumor progression of gastric cancer via the regulation of cell proliferation.
引用
收藏
页码:115 / 119
页数:5
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