Methods for using Galleria mellonella as a model host to study fungal pathogenesis

被引：258

作者：

Fuchs, Beth Burgwyn ^{[1
]}

O'Brien, Elizabeth ^{[1
]}

El Khoury, Joseph B. ^{[1
,2
,3
]}

Mylonakis, Eleftherios ^{[1
]}

机构：

[1] Massachusetts Gen Hosp, Div Infect, Boston, MA 02114 USA

[2] Massachusetts Gen Hosp, Ctr Immunol & Inflammatory Dis, Boston, MA 02114 USA

[3] Massachusetts Gen Hosp, Div Rheumatol Allergy & Immunol, Boston, MA 02114 USA

来源：

VIRULENCE | 2010年 / 1卷 / 06期

关键词：

filamentation; fungal pathogenesis; infection model; Galleria mellonella; model host; virulence; CANDIDA-ALBICANS; PATHOGENICITY; VIRULENCE; LARVAE;

D O I：

10.4161/viru.1.6.12985

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The facile inoculum delivery and handling of the insect Galleria mellonella make it a desirable model for the study of fungal pathogenesis. Here we present methods to study fungal virulence, filamentation and fungal cell associates with insect hemocytes using Candida albicans and Cryptococcus neoformans to illustrate the use of this model. The two types of fungi cause distinct infections; thus, we compare and contrast the infection characteristics observed in G. mellonella. The protocols presented herein can be adapted to the study of other fungal pathogens using G. mellonella as an infection model.

引用

页码：475 / 482

页数：8

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