Quality control limits for fluconazole disk susceptibility tests on Mueller-Hinton agar with glucose and methylene blue

被引:16
作者
Barry, A
Bille, J
Brown, S
Ellis, D
Meis, J
Pfaller, M
Rennie, R
Rinaldi, M
Rogers, T
Traczewski, M
机构
[1] Inst Clin Microbiol, Wilsonville, OR 97070 USA
[2] Univ Lausanne Hosp, Inst Microbiol, Lausanne, Switzerland
[3] Womens & Childrens Hosp, Adelaide, SA, Australia
[4] Canisius Wilhelmina Hosp, Ctr Infect Dis, Nijmegen, Netherlands
[5] Univ Iowa, Iowa City, IA USA
[6] Univ Alberta Hosp, Edmonton, AB T6G 2B7, Canada
[7] Univ Texas, Hlth Sci Ctr, San Antonio, TX USA
[8] Univ London Imperial Coll Sci Technol & Med, London, England
关键词
D O I
10.1128/JCM.41.7.3410-3412.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An international collaborative study was performed in order to propose quality control limits for fluconazole disk diffusion susceptibility tests on Mueller-Hinton agar with 2% glucose and 0.5 mug of methylene blue per ml. The supplements may be added before autoclaving the agar, or Mueller-Hinton agar plates may be Hooded with a glucos-methylene blue solution. Replicate tests on both types of agar plates generated data to propose zone size limits for tests of Candida parapsilosis ATCC 22019 (22 to 33 mm), C. tropicalis ATCC 750 (26 to 37 mm), and C. albicans ATCC 90028 (28 to 39 mm). C. krusei ATCC 6258 was not useful for this purpose.
引用
收藏
页码:3410 / 3412
页数:3
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