Biocatalytic preparation of N-glycolylneuraminic acid, deaminoneuraminic acid (KDN) and 9-azido-9-deoxysialic acid oligosaccharides

被引：27

作者：

Blixt, O ^{[1
]}

Paulson, JC ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

来源：

ADVANCED SYNTHESIS & CATALYSIS | 2003年 / 345卷 / 6-7期

关键词：

carbohydrates; enzyme catalysis; neuraminic acid; oligosaccharides; sialic acid; sialyltransferases;

D O I：

10.1002/adsc.200303032

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A facile approach for preparative synthesis of sialyloligosaccharides containing N-glycolylneuraminic acid, deaminoneuraminic acid, and two 9-azido derivatives is described. Synthesis is accomplished by use of 6-carbon mannose/mannosamine precursors employing an aldolase, CMP-sialic acid synthetase, and sialyltransferase. Using a combination of five different sialyltransferases and various acceptor substrates, 16 sialosides representing sequences found in glycoproteins and glycolipids were synthesized with typical yields of 60-80%.

引用

页码：687 / 690

页数：4

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